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Neuropediatrics 1988; 19(3): 158-161
DOI: 10.1055/s-2008-1052421
© Georg Thieme Verlag KG Stuttgart · New York

Evaluation of the Cytotoxicity of Sodium Valproate on Primary Cultured Rat Hepatocytes

T.  Takeuchi , T.  Sugimoto , N.  Nishida , Y.  Kobayashi
  • Department of Pediatrics, Kansai Medical University, Osaka, Japan
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Publication History

Publication Date:
19 March 2008 (online)

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Abstract

Primary cultured rat hepatocytes were used to study the cytotoxicity of sodium valproate (VPA). Cytotoxicity was monitored by measurement of leakage of intracellular enzymes into the culture medium: lactate dehydrogenase (LDH), glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT). The effects of D,L-carnitine and albumin administration on the cytotoxicity were evaluated.

LDH leakage rose with an increasing dose of VPA. Administrations of D,L-carnitine and albumin reduced VPA hepatotoxicity. Our data suggest that VPA-induced hepatotoxicity is dose-related and may be modulated by serum carnitine and albumin levels.

Key words

Hepatotoxicity - LDH - Valproate - Carnitine - Albumin - Rat

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