TY - JOUR AU - Malekinejad, Zahra; Aghajani, Shadi; Jeddi, Mostafa; Qahremani, Reihane; Shahbazi, Sina; Bagheri, Yasin; Ahmadian, Elham TI - Prazosin Treatment Protects Brain and Heart by Diminishing Oxidative Stress and Apoptotic Pathways After Renal Ischemia Reperfusion SN - 2194-9379 SN - 2194-9387 PY - 2022 JO - Drug Res (Stuttg) JF - Drug Research LA - EN VL - 72 IS - 06 SP - 336 EP - 342 DA - 2022/04/14 KW - Renal ischemia reperfusion KW - multi-organ dysfunction KW - oxidative stress KW - acute kidney injury KW - Prazosin AB - Acute kidney injury (AKI) is a major medical challenge caused from renal ischemia-reperfusion (IR) injury connected with different cellular events in other distant organs. Renal IR-related oxidative stress and inflammation followed by cell apoptosis play a crucial role in IR-induced distant organ pathological damages. Prazosin has shown protective effects against IR-injuries. Thus, the current study intended to investigate the possible protective role of prazosin against the consequents of renal IR in the heart and brain tissues. To reach this goal, rats were randomly divided into 3 groups (n=7): Sham, IR and prazosin pretreatment-IR animals (1 mg/kg intraperitoneally injection of prazosin 45 min before IR induction). After 6 h reperfusion, lipid peroxidation and antioxidant markers levels were evaluated in the both, brain and heart tissue. Moreover, apoptotic pathway in the heart and brain tissues were assessed by western blotting. Accordingly, prazosin pretreatment in IR model rats could significantly increase the antioxidant capacity and attenuate apoptotic pathways by increasing the bcl-2 levels and decreasing the expression of Bax and caspase 3 enzymes (P<0.05). Thus, prazosin suppressed cellular damages of heart and brain tissues post kidney IR by anti-oxidative and anti-apoptotic effects, which suggests the plausible use of prazosin in improving the clinical outcomes during AKI after further investigations. PB - Georg Thieme Verlag DO - 10.1055/a-1806-1453 UR - http://www.thieme-connect.de/products/ejournals/abstract/10.1055/a-1806-1453 ER -