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Thromb Haemost 1999; 81(04): 576-581
DOI: 10.1055/s-0037-1614527
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Schattauer GmbH

Increased Thrombogenic Potential of Human Monocyte-derived Macrophages Spontaneously Transformed into Foam Cells

Susanna Colli
1   From the Institute of Pharmacological Sciences, E. Grossi Paoletti Center, University of Milan, Milan, Italy
,
Mariagrazia Lalli
1   From the Institute of Pharmacological Sciences, E. Grossi Paoletti Center, University of Milan, Milan, Italy
,
Patrizia Risè
1   From the Institute of Pharmacological Sciences, E. Grossi Paoletti Center, University of Milan, Milan, Italy
,
Luciana Mussoni
1   From the Institute of Pharmacological Sciences, E. Grossi Paoletti Center, University of Milan, Milan, Italy
,
Sonia Eligini
1   From the Institute of Pharmacological Sciences, E. Grossi Paoletti Center, University of Milan, Milan, Italy
,
Claudio Galli
1   From the Institute of Pharmacological Sciences, E. Grossi Paoletti Center, University of Milan, Milan, Italy
,
Elena Tremoli
1   From the Institute of Pharmacological Sciences, E. Grossi Paoletti Center, University of Milan, Milan, Italy
› Author Affiliations
Further Information

Publication History

Received 04 November 1997

Accepted after resubmission 07 January 1999

Publication Date:
09 December 2017 (online)

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Summary

This study investigated whether spontaneous lipid enrichment of human macrophages affects their thrombogenic potential as measured by increased production of tissue factor (TF) and plasminogen activation inhibitor types 1 and 2 (PAI-1 and PAI-2). Macrophages were obtained following a 7-day culture period of monocytes, isolated from the same donor, in autologous serum (HS) or in fetal bovine serum (FBS). Those cultured in HS underwent marked lipid accumulation relative to those cultured in FBS that was accompanied by increased production of TF and PAI-1, but not of PAI-2, and decreased production of interleukin-1β. They also contained more arachidonic and linoleic acid and lower amounts of n-3 polyunsaturated fatty acids, particularly docosahexaenoic acid (22:6). These data indicate that the transformation of macrophages into foam cells results in an increase in their thrombogenic and antifibrinolytic potential and provide a possible explanation of the thrombotic sequelae frequently consequent on plaque fissuring and disruption.

 

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