Comparison of the Generation of Pancreatic Cancer Patient-Derived Organoids by Endoscopic Ultrasound-Guided fine Needle Aspirations and fine Needle Biopsies

Aims The prognosis for pancreatic cancer remains poor. Molecular diagnostics and customized therapies are becoming increasingly important in clinical routine. Patient-derived, predictive model systems such as organoids of endoscopic ultrasound (EUS)-guided punctures have the potential to substantially increase the depth of information from biopsy material by functional and molecular characterization of pancreatic cancer-patient derived organoids (PDOs). Improving the take rate of these organoids is a key step to bring this technology into routine clinical diagnostics.

To compare the extent to which the use of fine needle aspiration needles (FNA, 22G) or fine needle biopsy needles (FNB, 22G) influences the generation of pancreatic cancer patient-derived organoids to establish endoscopic standards of organoid technology.

Methods EUS-guided punctures by EUS-FNA and –FNB of pancreatic masses, highly suspicious for adenocarcinoma (detected by CT and/or MRI) were prospectively evaluated. Each patient received EUS-FNA and –FNB in a randomized order without the need to exchange the needle shaft (only the inner needle type (FNA/-B) was exchanged) between the passes. Per needle at least one passage for histology and one passage for PDOs were performed.

Results 50 patients were enrolled in the study. Histology revealed malignancy in 42 of 50 cases (84 %). In total 17 PDOs were generated. Of these 9 were established by FNB, 2 by FNA and 6 by both, FNA and FNB. In PDO cases histology revealed malignancy in 13 of 17 cases (76 %).

Conclusions EUS-FNB was superior to EUS-FNA in terms of successful generation of PDOs. Thus EUS-FNB might become the endoscopic standard to gain tissue for the evolving technology of organoids.

Citation: Wiessner JR, Orben F, Schäfer A et al. OP68 COMPARISON OF THE GENERATION OF PANCREATIC CANCER PATIENT-DERIVED ORGANOIDS BY ENDOSCOPIC ULTRASOUND-GUIDED FINE NEEDLE ASPIRATIONS AND FINE NEEDLE BIOPSIES. Endoscopy 2021; 53: S28.

Publication History

Article published online:
19 March 2021