Semin Thromb Hemost 2000; Volume 26(Number 01): 097-100
DOI: 10.1055/s-2000-9810
Copyright © 2000 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA. Tel.: +1(212) 584-4663)

Whole Blood Prothrombin Time Using Diluted Tissue Factor is Shortened in Spontaneous Hypertensive Rats

Machiko Munakata1 , Yutaka Komiyama1 , Midori Masuda1 , Hideo Kagawa2 , Shosaku Nomura2 , Shirou Fukuhara2 , Hakuo Takahashi1
  • Supported in part by a grant-in-aid for scientific research from the Japanese Ministry of Education, Science and Culture.
  • 1Department of Clinical Sciences and Laboratory Medicine, Kansai Medical University, Moriguchi, Osaka, Japan
  • 2The First Department of Internal Medicine, Kansai Medical University, Moriguchi, Osaka, Japan
Further Information

Publication History

Publication Date:
31 December 2000 (online)

 

ABSTRACT

Clinical screening tests for blood coagulation that use plasma as samples cannot estimate the participation of platelets, leukocytes, and erythrocytes in blood coagulation system. We developed an assay to evaluate the total coagulation ability of blood and whole blood prothrombin time (WPT) using the principle of prothrombin time with the diluted-tissue factor as a trigger and a newly developed apparatus, STA, viscosity change detection system (electromagnetic clot detection). The activation of platelets by Ca ionophore shortened WPT and increased the expression of CD62P on the platelet surface. WPTs in citrated blood of spontaneous hypertensive rats (SHR) were significantly shorter than those of controls, Wistar Kyoto rats (WKY). Plasma levels of thrombin-antithrombin III (TAT) in SHR were significantly higher than those of WKY. Moreover, WPT and TAT levels were significantly correlated. Based on these results, WPT was found to be useful to estimate the activation of blood coagulation in whole blood.

KEYWORD

Prothrombin time - whole blood - tissue factor - hypertension

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