Enhanced nutritionally induced adipose tissue development in mice with stromelysin-1 gene inactivation

Erik Maquoi; Diego Demeulemeester; Gabor Vörös; Désire Collen; H. Roger Lijnen

doi:10.1055/s-0037-1613586

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2003; 89(04): 696-704
DOI: 10.1055/s-0037-1613586

Cellular Proteolysis and Oncology

Schattauer GmbH

Enhanced nutritionally induced adipose tissue development in mice with stromelysin-1 gene inactivation

Autor*innen

Erik Maquoi

¹Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
Diego Demeulemeester

¹Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
Gabor Vörös

¹Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
Désire Collen

¹Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
H. Roger Lijnen

¹Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium

Abstract

Summary

To investigate a potential role of stromelysin-1 (MMP-3) in development of adipose tissue, 5 week old male MMP-3 deficient mice (MMP-3^-/-) and wild-type (MMP-3^+/+) controls were kept on a high fat diet (HFD) for 15 weeks. MMP-3^-/- mice were hyperphagic and gained more weight than the MMP-3^+/+ mice. At the time of sacrifice, the body weight of the MMP-3^-/- mice was significantly higher than that of the MMP-3^+/+ mice, as was the weight of the isolated subcutaneous (SC) and gonadal (GON) fat deposits. Significant adipocyte hypertrophy was observed in the GON but not in the SC adipose tissue of MMP-3-/- mice. Fasting plasma glucose and cholesterol levels were comparable in both genotypes, whereas triglyceride levels were significantly lower in MMP-3^-/- mice. Staining with an endothelial cell specific lectin revealed a significantly higher blood vessel density and larger total stained area in the GON adipose tissues of MMP-3^-/- mice. Thus, in a murine model of nutritionally induced obesity, MMP-3 impairs adipose tissue development, possibly by affecting food intake and/or adipose tissue-related angiogenesis.

Theme paper: Part of this paper was originally presented at the joint meetings of the 16th International Congress of the International Society of Fibrinolysis and Proteolysis (ISFP) and the 17th International Fibrinogen Workshop of the International Fibrinogen Research Society (IFRS) held in Munich, Germany, September, 2002.

Keywords

MMP-3 - stromelysin-1 - obesity - adipose tissue - adipocyte - angio-genesis

Volltext

Referenzen

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