Summary
The subendothelial surface of rabbit aorta and α chymotrypsin-digested subendothelium
were exposed to anticoagulated human blood in an annular flow chamber. The wall shear
rate was similar to that observed in large arteries (830 sec–1) and exposure times varied from 2½ to 40 min.
The platelet reactive substrate of α chymotrypsin-digested subendothelium consists
of a three-dimensional meshwork of collagen fibrils which form islands of variable
size and height in a matrix of virtually unreactive elastin. Collagen-induced aggregation
in the aggregometer was similar with or without prior α chymotrypsin-digestion of
a highly dispersed preparation of fibrillar collagen. The rate of platelet adhesion
was decreased on the fibrillar collagen of α chymotrypsin-digested subendothelium
as compared to intact subendothelium. On the other hand the rate of aggregation was
increased once platelets adhered to the fibrillar collagen. Mural thrombi (aggregates)
disappeared on subendothelium whereas they grew progressively on the fibrillar collagen.
Thus the fibrillar collagen of α chymotrypsin-digested subendothelium appears to be
a more thrombogenic surface. It is suggested that physical (loose three-dimensional
meshwork versus a comparatively solid surface) and/or chemical (number of platelet
reactive sites per unit surface area) differences between the two surfaces may explain
the platelet-surface-interaction patterns which are characteristic for each surface.
