Contribution of a New, Rapid, Individual and Quantitative Automated D-Dimer ELISA to Exclude Pulmonary Embolism

Philippe de Moerloose; Sylvie Desmarais; Henri Bounameaux; Guido Reber; Arnaud Perrier; Georges Dupuy; Jean-Louis Pittet

doi:10.1055/s-0038-1650213

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1996; 75(01): 011-013
DOI: 10.1055/s-0038-1650213

Original Article

Schattauer GmbH Stuttgart

Contribution of a New, Rapid, Individual and Quantitative Automated D-Dimer ELISA to Exclude Pulmonary Embolism

Authors

Philippe de Moerloose

¹The Division of Angiology and Haemostasis, University Hospital of Geneva, Switzerland
Sylvie Desmarais

¹The Division of Angiology and Haemostasis, University Hospital of Geneva, Switzerland
Henri Bounameaux

¹The Division of Angiology and Haemostasis, University Hospital of Geneva, Switzerland
Guido Reber

¹The Division of Angiology and Haemostasis, University Hospital of Geneva, Switzerland
Arnaud Perrier

²The Medical Clinics 1, University Hospital of Geneva, Switzerland
Georges Dupuy

³The Research Laboratories bioMérieux, Marcy l’Etoile, France
Jean-Louis Pittet

³The Research Laboratories bioMérieux, Marcy l’Etoile, France

Abstract

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Summary

The performance of a new automated ELISA for a rapid, individual and quantitative measurement of plasma D-dimer (VIDAS D-dimer) has been evaluated. First, a study of 100 patients was performed in order to choose the best couple of antibodies in comparison with an already clinically validated ELISA. Then the results were certified in a prospective study including 195 consecutive patients suspected of pulmonary embolism (PE). For a cut-off level of 500 ng/ml VIDAS D-dimer showed a sensitivity of 100% (95% confidence interval 92-100), a specificity of 37.6%, a negative predictive value of 100% (95% CI 93.3-100) and a positive predictive value of 33.1%. During a 6 months’ follow-up no patient (95% CI 0-6.4) with D-dimer <500 ng/ml presented a new suspicion of venous thromboembolic disease. These results suggest that this rapid and single-dose ELISA provides a very useful tool for the clinician to exclude on a day-to-day basis the diagnosis of PE.

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Referenzen

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