Indium-111-Labelled Human Platelets: A Method for Use in Severe Thrombocytopenia

A du P Heyns; P N Badenhorst; P Wessels; H Pieters; H F Kotzè; M G Lötter

doi:10.1055/s-0038-1661183

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1984; 52(03): 226-229
DOI: 10.1055/s-0038-1661183

Original Article

Schattauer GmbH Stuttgart

Indium-111-Labelled Human Platelets: A Method for Use in Severe Thrombocytopenia

Authors

A du P Heyns

The M.R.C. Blood Platelet Research Unit, University of the Orange Free State, Bloemfontein, South Africa
P N Badenhorst

The M.R.C. Blood Platelet Research Unit, University of the Orange Free State, Bloemfontein, South Africa
P Wessels

The M.R.C. Blood Platelet Research Unit, University of the Orange Free State, Bloemfontein, South Africa
H Pieters

The M.R.C. Blood Platelet Research Unit, University of the Orange Free State, Bloemfontein, South Africa
H F Kotzè

The M.R.C. Blood Platelet Research Unit, University of the Orange Free State, Bloemfontein, South Africa
M G Lötter

The M.R.C. Blood Platelet Research Unit, University of the Orange Free State, Bloemfontein, South Africa

Abstract

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Summary

We describe and evaluate a simple method for labelling autologous human platelets with Indium-111-oxine in patients with severe thrombocytopenia. Twenty patients with immune thrombocytopenia and platelet counts ranging from 5 to 119 × 10⁹/1 were investigated. Platelets were isolated from blood by differential centrifugation, residual platelets were repeatedly washed from the red cell layer and buffy coat and labelled with In 111 in saline. A mean of 55% ± 21 of platelets were harvesteo from the blood, labelled with 49% ± 24 efficiency and 15.8 × 108 labelled platelets reinjected to the patients. Contamination of the platelets with red cells and plasma was low. The labelled platelets were viable as assessed by in vitro aggregation, recovery in the circulation and mean survival time. This method permits quantitative platelet imaging with autologous labelled platelets in patients with severe thrombocytopenia.

Key words

Indium-111-labelled platelets - Platelet labelling in thrombocytopenia - Platelet label method

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Referenzen

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