The influence of sodium ion binding on factor IXa activity

Kota Gopalakrishna; Alireza R. Rezaie

doi:10.1160/TH06-03-0156

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2006; 95(06): 936-941
DOI: 10.1160/TH06-03-0156

Blood Coagulation, Fibrinolysis and Cellular Haemostasis

Schattauer GmbH

The influence of sodium ion binding on factor IXa activity

Autor*innen

Kota Gopalakrishna

¹Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St. Louis, Missouri, USA
Alireza R. Rezaie

¹Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St. Louis, Missouri, USA

Abstract

Summary

Sodium plays an important role in modulating both the amidolytic and proteolytic activities of thrombin. By contrast, while the optimal amidolytic activity of factor Xa requires Na⁺, the proteolytic activity of factor Xa in the prothrombinase complex is minimally affected by the monovalent cation. In this study, we analyzed the effect of Na⁺ on the amidolytic and proteolytic activity of factor IXa in the absence and presence of factor VIIIa. Factor IXa exhibited normal activity towards a fIXa-specific chromogenic substrate and antithrombin in the presence of physiological concentrations of Ca²⁺ with no obvious requirement for Na⁺ in either reaction. Further studies revealed that factor IXa binds to its cofactor factor VIIIa with a normal affinity in the absence of Na⁺ and that the catalytic function in the intrinsic Xase complex is also independent of Na⁺ in the presence of physiological concentrations of Ca²⁺.These results suggest that unlike the important role that Na⁺ plays in modulating the macromolecular substrate specificity of thrombin, the monovalent cation is not required for the physiological function of factor IXa in the intrinsic Xase complex.

Keywords

Factor IXa - factor X - intrinsic Xase - sodium - calcium

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Referenzen

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