Multiple electrode aggregometry: A new device to measure platelet aggregation in whole blood

Orsolya Tóth; Andreas Calatzis; Sandra Penz; Hajna Losonczy; Wolfgang Siess

doi:10.1160/TH06-05-0242

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2006; 96(06): 781-788
DOI: 10.1160/TH06-05-0242

Platelets and Blood Cells

Schattauer GmbH

Multiple electrode aggregometry: A new device to measure platelet aggregation in whole blood

Orsolya Tóth

¹Institute for Prevention of Cardiovascular Diseases, University of Munich, Munich, Germany

²1^stDepartment of Medicine, University of Pécs, Pécs, Hungary

,

Andreas Calatzis

³Department of Transfusion Medicine and Hemostaseology, University of Munich, Munich, Germany

,

Sandra Penz

¹Institute for Prevention of Cardiovascular Diseases, University of Munich, Munich, Germany

,

Hajna Losonczy

²1^stDepartment of Medicine, University of Pécs, Pécs, Hungary

,

Wolfgang Siess

¹Institute for Prevention of Cardiovascular Diseases, University of Munich, Munich, Germany

› Author Affiliations

Abstract

Summary

Several methods are used to analyse platelet function in whole blood. A new device to measure whole blood platelet aggregation has been developed, called multiple electrode platelet aggregometry (MEA). Our aim was to evaluate MEA in comparison with the single platelet counting (SPC) method for the measurement of platelet aggregation and platelet inhibition by aspirin or apyrase in diluted whole blood. Platelet aggregation induced by different concentrations of ADP, collagen and TRAP-6 and platelet inhibition by apyrase or aspirin were determined in citrateor hirudin-anticoagulated blood by MEA and SPC. MEA indicated that spontaneous platelet aggregation was lower, and stimulated platelet aggregation was higher in hirudin- than citrate-anticoagulated blood. In hirudin-anticoagulated, but not citrate-anticoagulated blood, spontaneous platelet aggregation measured by MEA was inhibited by apyrase. For MEA compared with SPC the dose response-curves of agonist-induced platelet aggregation in citrate- and hirudin-blood showed similar EC50 values for TRAP, and higher EC50 values for ADP (non-significant) and collagen (p<0.05). MEA and the SPC method gave similar results concerning platelet-inhibition by apyrase and aspirin. MEA was more sensitive than SPC to the inhibitory effect of aspirin in collagen-induced aggregation. In conclusion, MEA is an easy, reproducible and sensitive method for measuring spontaneous and stimulated platelet aggregation, and evaluating antiplatelet drugs in diluted whole blood. The use of hirudin as an anticoagulant is preferable to the use of citrate. MEA is a promising technique for experimental and clinical applications.

Keywords

Anticoagulation - multiple electrode aggregometry - platelet aggregation - single platelet counting

Full Text

References

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