Effects of homeopathic medications Eupatorium perfoliatum and Arsenicum album on parasitemia of Plasmodium berghei-infected mice

G. Lira-Salazar; E. Marines-Montiel; J. Torres-Monzón; F. Hernández-Hernández; J.S. Salas-Benito

doi:10.1016/j.homp.2006.06.003

Homeopathy, Table of Contents

Homeopathy 2006; 95(04): 223-228
DOI: 10.1016/j.homp.2006.06.003

Original Paper

Effects of homeopathic medications Eupatorium perfoliatum and Arsenicum album on parasitemia of Plasmodium berghei-infected mice

Authors

G. Lira-Salazar

¹Especialización en Terapéutica Homeopática
E. Marines-Montiel

¹Especialización en Terapéutica Homeopática
J. Torres-Monzón

³Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados of Instituto Politécnico Nacional, Mexico
F. Hernández-Hernández

³Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados of Instituto Politécnico Nacional, Mexico
J.S. Salas-Benito

²Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía of Instituto Politécnico Nacional

Subject Editor:

Abstract

Malaria is one of the most important parasitic diseases in the world and a major public health problem because of emerging drug-resistant strains of Plasmodium. A number of synthetic and natural compounds are now being analysed to develop more effective antimalarial drugs. We investigated the effect of homeopathic preparations of Eupatorium perfoliatum and Arsenicum album on parasitemia using a rodent malaria model.

We found significant inhibitory effect on parasite multiplication with both medications with a level of 60% for Eupatorium perfoliatum at a 30 CH potency. Arsenicum album 0/6 gave 70% inhibition but this was less stable than Eupatorium perfoliatum. The number of schizonts was higher in animals treated with homeopathic medications. Although the mechanism of action is unknown, these agents would be good candidates as alternative or complementary medications in the treatment of malaria.

Keywords

Plasmodium berghei - Eupatorium perfoliatum - Arsenicum album - Balb/c mice - Homeopathic medications

Full Text

References

References
1 Breman J.G. The ears of the hippopotamus: manifestations, determinants, and estimates of the malaria burden. Am J Trop Med Hyg 2001; 64 (Suppl. 01) 1-11.
2 Ang K.K.H., Holmes M.J., Higa T., Hamann M.T., Kara U.A.K. In vivo antimalarial activity of the beta-carboline alkaloid manzamine A. Antimicrob Agents Chemother 2000; 44: 1645-1649.
3 Darkin-Rattray S.J., Gurnett A.M., Myers R.W. et al. Apicidin: a novel antiprotozoal agent that inhibits parasite histone deacetylase. Proc Natl Acad Sci 1996; 93: 13143-13147.
4 Ridley R.G., Hofheinz W., Matile H. et al. 4-aminoquinoline analogs of chloroquine with shortened side chains retain activity against chloroquine-resistant Plasmodium falciparum . Antimicrob Agents Chemother 1996; 40: 1846-1854.
5 Vennerstrom J.L., Ager A.L., Andersen S.L. et al. Assessment of the antimalarial potential of tetraoxane WR 148999. Am J Trop Med Hyg 2000; 62: 573-578.
6 Delarue S., Girault S., Dali Ali F., Maes L., Grellier P., Sergheraert C. “One pot” synthesis and antimalarial activity of formamidine derivatives of 4-anilinoquinoline. Chem Pharm Bull 2001; 49: 933-937.
7 Vennerstrom J.L., Arbe-Barnes S., Brun R. et al. Identification of an antimalarial synthetic trioxolane drug development candidate. Nature 2004; 430: 900-904.
8 de las Heras B., Slowing K., Benedi J. et al. Antiinflammatory and antioxidant activity of plants used in traditional medicine in Ecuador. J Ethnopharmacol 1998; 61: 161-166.
9 Clavin M.L., Gorzalczany S., Mino J. et al. Antinociceptive effect of some Argentine medicinal species of Eupatorium . Phytother Res 2000; 14: 275-277.
10 Muschietti L., Gorzalczany S., Ferraro G., Acevedo C., Martino V. Phenolic compounds with anti-inflammatory activity from Eupatorium buniifolium . Planta Med 2001; 67: 743-744.
11 Mino J., Muschietti L., Ferraro G., Martino V., Acevedo C. Antinociceptive activity of Eupatorium buniifolium aqueous extract. Fitoterapia 2005; 76: 100-103.
12 Caceres A., Menendez H., Mendez E. et al. Antigonorrhoeal activity of plants used in Guatemala for the treatment of sexually transmitted diseases. J Ethnopharmacol 1995; 48: 85-88.
13 Urzua A., Caroli M., Vasquez L., Mendoza L., Wilkens M., Tojo E. Antimicrobial study of the resinous exudate and of diterpenoids isolated from Eupatorium salvia (Asteraceae). J Ethnopharmacol 1998; 62: 251-254.
14 Abad M.J., Bermejo P., Sanchez Palomino S., Chiriboga X., Carrasco L. Antiviral activity of some South American medicinal plants. Phytother Res 1999; 13: 142-146.
15 Hnatyszyn O., Broussalis A., Herrera G. et al. Argentine plant extracts active against polymerase and ribonuclease H activities of HIV-1 reverse transcriptase. Phytother Res 1999; 13: 206-209.
16 Zanon S.M., Ceriatti F.S., Rovera M., Sabini L.J., Ramos B.A. Search for antiviral activity of certain medicinal plants from Cordoba, Argentina. Rev Latinoam Microbiol 1999; 41: 59-62.
17 El-Seedi H.R., Ohara T., Sata N., Nishiyama S. Antimicrobial diterpenoids from Eupatorium glutinosum (Asteraceae). J Ethnopharmacol 2002; 81: 293-296.
18 Gupta M., Mazumder U.K., Chaudhuri I. et al. Antimicrobial activity of Eupatorium ayapana . Fitoterapia 2002; 73: 168-170.
19 Garcia C.C., Talarico L., Almeida N., Colombres S., Duschatzky C., Damonte E.B. Virucidal activity of essential oils from aromatic plants of San Luis, Argentina. Phytother Res 2003; 17: 1073-1075.
20 Navarro Garcia V.M., Gonzalez A., Fuentes M. et al. Antifungal activities of nine traditional Mexican medicinal plants. J Ethnopharmacol 2003; 87: 85-88.
21 Rios M.Y., Aguilar-Guadarrama A.B., Navarro V. Two new benzofuranes from Eupatorium aschenbornianum and their antimicrobial activity. Planta Med 2003; 69: 967-970.
22 Sasikumar J.M., Doss A.P., Doss A. Antibacterial activity of Eupatorium glandulosum leaves. Fitoterapia 2005; 76: 240-243.
23 Blair S., Mesa J., Correa A., Carmona-Fonseca J., Granados H., Saez J. Antimalarial activity of neurolenin B and derivates of Eupatorium inulaefolium (Asteraceae). Pharmazie 2002; 57: 413-415.
24 Carvalho L.H., Brandao M.G., Santos-Filho D., Lopes J.L., Krettli A.U. Antimalarial activity of crude extracts from Brazilian plants studied in vivo in Plasmodium berghei-infected mice and in vitro against Plasmodium falciparum in culture. Braz J Med Biol Res 1991; 24: 1113-1123.
25 Habtemariam S., Macpherson A.M. Cytotoxicity and antibacterial activity of ethanol extract from leaves of a herbal drug, boneset (Eupatorium perfoliatum). Phytother Res 2000; 14: 575-577.
26 Gassinger C.A., Wunstel G., Netter P. A controlled clinical trial for testing the efficacy of the homeopathic drug Eupatorium perfoliatum D2 in the treatment of common cold. Arzneimittelforschung 1981; 31: 732-736.
27 Mendiola-Quezada R. Arsenicum album . in: Adalpe Barrera F. Farmacodinamia Homeopática. 1987. México: UNAM; 77-81.
28 Tona L., Mesia K., Ngimbi N.P. et al. In vivo antimalarial activity of Cassia occidentalis, Morinda morindoides and Phyllanthus niruri . Ann Trop Med Parasitol 2001; 95: 47-57.
29 Sandoval L.G. Farmacopea Homeopatica Mexicana. 3rd edn. New Delhi: B Jain Publishers; 1996. pp 18, 26–27, 32–33, 90, 188–189.
[30] Hahnemann S. Organon de la medicina. 7th ed. Mexico D.F.: Porrua Editorial; 2002. pp. 255–259.
31 Su R.B., Wei X.L., Liu Y., Li J. Antimalarial effect of agmantine on Plasmodium berghei K173 strain. Acta Pharmacol Sin 2003; 24: 918-922.
32 Fry J. One way analysis of variance. in: Fry J. Biological Data Analysis. A Practical Approach Series. 1996. Oxford: Oxford University Press; 1-11.
33 Hou L.J., Raju S., Abdullah M.S., Nor N.M., Ravichandran M. Rifampicin antagonizes the effect of chloroquine on chloroquine-resistant Plasmodium beghei in mice. Jpn J Infect Dis 2004; 57: 198-202.
34 Franssen F.F.J., Smeijsters L.J.J.W., Berger I., Mendinilla Aldana B.E. In vivo and In vitro atiplasmodial activities of some plants traditionally used in Guatemala against malaria. Antimicrob Agents Chemother 1997; 41: 1500-1503.
35 Wagner H., Jurcic K. Immunologic studies of plant combination preparations. In vitro and in vivo studies on the stimulation of phagocytosis. Arzneimittelforschung 1991; 41: 1072-1076.
36 Wagner H., Proksch A., Riess-Maurer I. et al. Immunostimulant action of polysaccharides (heteroglycans) from higher plants. Preliminary communication. Arzneimittelforschung 1984; 34: 659-661.
37 Perez H.A., De la Rosa M., Apitz R. In vivo activity of ajoene against rodent malaria. Antimicrob Agents Chemother 1994; 38: 337-339.
38 Bitonti A.J., Dumont J.A., Bush T.L. et al. Bis(benzyl)polyamine analogs inhibit the growth of chloroquine-resistant human malaria parasites (Plasmodium falciparum) in vitro and in combination with a-difluoromethylornithine cure murine malaria. Proc Natl Acad Sci 1989; 86: 651-655.
39 Deharo E., Barkan D., Krugliak M., Golenser J., Ginsburg H. Potentiation of the antimalarial action of chloroquine in rodent malaria by drugs known to reduce cellular glutathione levels. Biochem Pharmacol 2003; 66: 809-817.
40 Berberian D.A., Slighter R.G. Chemotherapeutic activity of combination doses of chloroquine, pyrimethamine, and sulfamethoxydiazine, a long-acting sulfanilamide, against Plasmodium berghei infections in mice. Antimicrob Agents Chemother 1973; 3: 392-498.
41 Wang J.Y., Cao W.C., Shan C.Q. et al. Naphthoquine phosphate and its combination with artemisinine. Acta Trop 2004; 89: 375-381.