Lipid peroxidation, erythrocyte antioxidants and plasma antioxidants in osteoarthritis before and after homeopathic treatment

Objective: This study attempts to evaluate the status of oxidative stress in osteoarthritis (OA), by measuring some parameters of oxidant stress and antioxidant defenses in blood, before and after homeopathy treatment, and to asses the role, if any, of homeopathic treatment in modulating free radical toxicity in OA.

Methods: Erythrocyte lipid peroxidation (LP), erythrocyte antioxidants viz., glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD), catalase (CT) and plasma antioxidants viz., ceruloplasmin, glutathione-S-transferase (GST), vitamin C, total antioxidant activity (AOA) were determined in eighty one patients with OA and fifty three normals. Forty seven patients, who were treated with homeopathic remedies were considered for the follow-up studies.

Location: Father Muller Homeopathic Hospital, Mangalore, South Karnataka, India.

Results: Erythrocyte LP (0 hour, p < 0.001; 2 hours, p < 0.01; and susceptibility to LP, p < 0.05) and SOD (p < 0.05) were significantly higher, whereas plasma vitamin C (p < 0.01) and AOA (p < 0.001) were significantly lower in OA patients when compared to controls. In follow-up patients the erythrocyte LP (0 hour, p < 0.01; 2 hours, p < 0.01; and susceptibility to LP, p < 0.01) and SOD (p < 0.01) were significantly lower when compared to their pretreatment values. Plasma vitamin C attained a normal range. The AOA activity after treatment was not significantly different from that observed before treatment.

Conclusion: Oxidative stress increased in OA as indicated by increased LP, SOD, decreased vitamin C and AOA. On homeopathic treatment the LP has decreased in the erythrocytes which shows and reduced oxidative stress. This is further evidenced by returning of plasma vitamin C and erythrocyte SOD to the normal levels, but oxidant stress has not been completely overcome as plasma AOA remained low after treatment.

Keywords

Free radicals - Homeopathy - Osteoarthritis

• References

• 1 Lockie A., Geddes N. In: Blanche S. The Complete Guide to Homeopathy. 1995. London, NewYork: Dorling Kindersley; 11-19.
• 2 Lockie A. In: Stephanic F. Encyclopedia of Homeopathy. 2000. London, NewYork: Dorling Kindersley; 12-25.
• 3 Long L., Ernst E. Homeopathic remedies for the treatment of osteoarthritis: a systamatic review. British Homeopathic Journal 2001; 90: 37-43.
• 4 Ostalwska A., Birkner E., Wiecha M. et al. Lipid peroxidation and antioxidant enzymes in synovial fluid of patients with primary and secondary osteoarthritis of the knee joint. Osteoarthritis Cartilage 2006; 14 (02) 139-145.
• 5 Henrotin Y., Kurz B. Antioxidant to treat osteoarthritis: dream or reality?. Curr Drug Targets 2007; 8 (02) 347-357.
• 6 Martin J.A., Brown T.D., Heiner A.D. et al. Chondrocyte senescence, joint loading and osteoarthritis. Clin Orthop 2004; 427: s96-s103.
• 7 Henrotin Y.E., Bruckner P., Pujol J.P. The role of reactive oxygen species in homeostasis and degradation of cartilage. Osteoarthritis Cartilage 2003; 11 (10) 747-755.
• 8 Henrotin Y.E., Kurz B., Aigner T. Oxygen and reactive oxygen species in cartilage degradation: friends or foes?. Osteoarthritis Cartilage 2005; 13 (08) 643-654.
• 9 Kartha V.N., Krishnamurthy S. Factors affecting in vitro lipid peroxidation of rat brain homogenate. Ind J Physiol Pharmacol 1978; 22: 44-52.
• 10 Stocks J., Dormandy T.L. The auto-oxidation of human red cell lipids induced by hydrogen peroxide. B J Haematol 1971; 20: 95-111.
• 11 Horn H.D., Burns F.H. Glutathione reductase. In: Bergmeyer H.V. Methods of Enzymatic Analysis. 1978. NewYork: Academic Press; 875
• 12 Beauchamp C., Fridrovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 1971; 44: 276-287.
• 13 Brannan T.S., Maker H.S., Raes I.P. Regional distribution of catalase in the adult rat brain. J Neurochem 1989; 36: 307.
• 14 Beutler E., Duron O., Kelly B.M. Improved method for the determination of blood glutathione. J Lab Clin Med 1963; 61: 882.
• 15 William S.F., Shozo N. Measurement of Serum ceruloplasmin by its p phenylenediamine oxidase activity. Clinical Chemistry 1970; 16 (11) 903-909.
• 16 Norbert WT. In: Nobert WT editor. Text book of Clinical Chemistry, 1986: 960.
• 17 Adachi Y., Horii K., Takahashi Y. et al. Serum glutathione-S-transferase activity in liver diseases. Clin Chim Acta 1980; 106: 243-255.
• 18 Koracevic D., Koracevic G., Djordjevic V. et al. Method for the measurement of antioxidant activity in human fluid. J Clin Pathol 2001; 54: 356-361.
• 19 Bonner W.M., Jonsson H., Malanos C., Bryant M. Changes in the lipids of human articular cartilage with age. Arthritis Rheum 1975; 18: 461-473.
• 20 Tiku M.L., Shah R., Allison G.T. Evidence linking chondrocyte lipid peroxidation to cartilage matrix protein degradation. J Biol Chem 2000; 275: 20069-20076.
• 21 Rubyk B.I., Filchang N.M., Sabadyshin R.A. Change in lipid peroxidation in patients with primary osteoarthrosis deformans. Ter Arkh 1988; 60 (09) 110-113.
• 22 Maneesh M., Jayalekshmi H., Suma T. et al. Evidence for oxidative stress in Osteoarthritis. Indian J of Clinical Biochemistry 2005; 20 (01) 129-130.
• 23 Deahl S.T., Oberley L.W., Oberley T.D., Elwell J.H. Immunohistochemical identification of superoxide dismutases, catalase, and glutathione-S-transferases in rat femora. J Bone Miner Res 1992; 7: 187-198.
• 24 Ivanova E., Ivanova B. Mechanisms of extacellular antioxidant defence. Exp Pathol 2000; 4: 49-59.
• 25 Schmacher HR. Synovial fluid analysis and synovial biopsy. In: Schmacher HR editor. Textbook of Rheumatology. 1997:609–625.
• 26 Sarban S., Kocyigit A., Yazar M., Isikan U.E. Plasma total antioxidant capacity, lipid peroxidation, and erythrocyte antioxidant enzyme activities in patients with rheumatoid arthritis and osteoarthritis. Clin Biochem 2005; 38 (11) 981-986.
• 27 Alpaslan C., Bilgihan A., Alpaslan G.M. et al. Effect of arthrocenesis and sodium hyaluronate injection on nitrite, nitrate and thiobarbituric acid–reactive substance levels in the synovial fluid. Oral Surg 2000; 89: 686-690.
• 28 Carlo M.D., Loeser R.F. Increased oxidative stress with aging reduces chondrocyte survival: correlation with intracellular glutathione levels. Arthritis Rheum 2003; 48: 3419-3430.