Homeopathy 2011; 100(04): 220-227
DOI: 10.1016/j.homp.2011.05.007
Original Paper
Copyright © The Faculty of Homeopathy 2011

Ultra High Dilution of triiodothyronine modifies cellular apoptosis in Rana catesbeiana tadpole tail in vitro

J.R.P. Guedes
1   Laboratory of Molecular Pathology, Department of Pathology, University of São Paulo School of Medicine, SP, Brazil
S. Carrasco
2   Discipline of Rheumatology, School of Medicine, Clinics Hospital, University of São Paulo, Brazil
C.M. Ferreira
3   Agricultural Department of São Paulo State, Aquaculture Research Center, Fishery Institute, Brazil
L.V. Bonamin
4   Research Center, University Paulista, Brazil
5   Laboratory of Veterinary Pathology, University of Santo Amaro, Brazil
W. Souza
1   Laboratory of Molecular Pathology, Department of Pathology, University of São Paulo School of Medicine, SP, Brazil
C. Goldenstein-Schainberg
2   Discipline of Rheumatology, School of Medicine, Clinics Hospital, University of São Paulo, Brazil
E.R. Parra
1   Laboratory of Molecular Pathology, Department of Pathology, University of São Paulo School of Medicine, SP, Brazil
V.L. Capelozzi
1   Laboratory of Molecular Pathology, Department of Pathology, University of São Paulo School of Medicine, SP, Brazil
› Author Affiliations

Subject Editor:
Further Information

Publication History

Received14 June 2010
revised27 April 2011

accepted06 May 2011

Publication Date:
20 December 2017 (online)

Background: Ultra High Dilutions (UHD) are diluted beyond the Avogadro limit with dynamization (dilution with succussion). The process of anuran amphibian metamorphosis is controlled by thyroid hormones, including the resorption of the tadpole tail.

Methods: A randomized and blinded study was performed to investigate the influence of triiodothyronine (T3) 5 · 10−24 M (10cH) on apoptosis induced by T3 100 nM in Rana catesbeiana tadpoles’ tail tips, in vitro. Explants were randomized to three groups: control: no T3 in pharmacological or UHD dose; test: T3 100 nM and challenged with T3 10cH (UHD); positive control: T3 100 nM, treated with unsuccussed ethanol. The apoptotic index and the area of explants of test and control groups at the first and final day of the experiment were compared by t-test.

Results: There was no difference in tail tip area between test and control groups, but a significantly higher (p < 0.01) index of apoptosis in explants of the test group.

Conclusion: This data suggest that T3 10cH modifies the effect of T3 at pharmacological dose, opening new perspectives for further studies and investigation of the dose–effect curve.

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