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Effect of the oral administration homeopathic Arnica montana on mitochondrial oxidative stress
Received09 December 2011
revised22 October 2012
accepted07 November 2012
17 December 2017 (online)
Objective: To analyze the effect of homeopathic Arnica on mitochondrial oxidative stress induced by Ca2+ plus inorganic phosphate and/or Fe2+-citrate-mediated lipid peroxidation through changes in oxygen consumption rates.
Methods: Mitochondria were isolated by differential centrifugation from the livers of adult male Wistar rats which had been treated with Arnica montana 6cH, 12cH, 30cH or succussed 30% ethanol (control) for 21 days.
Results: In the presence of antimycin-A, electron transport chain inhibitor, as evidenced by antimycin-A insensitive O2 consumption, Arnica inhibited lipid peroxidation of mitochondrial membranes. In oxidative stress conditions, in the presence of Ca2+ and inorganic phosphate, animals receiving Arnica 30cH had a significant decrease in mitochondrial O2 consumption compared to control animals.
Conclusion: When administrated orally, Arnica 30cH protects against hepatic mitochondrial membrane permeabilization induced by Ca2+ and/or Fe2+-citrate-mediated lipid peroxidation and fragmentation of proteins due to the attack by reactive oxygen species.
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