Effect of homeopathic preparations of Syzygium jambolanum and Cephalandra indica on gastrocnemius muscle of high fat and high fructose-induced type-2 diabetic rats

Sathish Sampath; Akilavalli Narasimhan; Raveendar Chinta; K.R. Janardanan Nair; Anil Khurana; Debadatta Nayak; Alok Kumar; Balasubramanian Karundevi

doi:10.1016/j.homp.2013.05.002

Homeopathy, Table of Contents

Homeopathy 2013; 102(03): 160-171
DOI: 10.1016/j.homp.2013.05.002

Original Paper

Effect of homeopathic preparations of Syzygium jambolanum and Cephalandra indica on gastrocnemius muscle of high fat and high fructose-induced type-2 diabetic rats

Authors

Sathish Sampath

¹Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Sekkizhar Campus, Taramani, Chennai 600 113, India
Akilavalli Narasimhan

¹Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Sekkizhar Campus, Taramani, Chennai 600 113, India
Raveendar Chinta

²Central Council for Research in Homeopathy, 61-65, Institutional Area, Opp. D-Block, Janakpuri, New Delhi 110 058, India
K.R. Janardanan Nair

²Central Council for Research in Homeopathy, 61-65, Institutional Area, Opp. D-Block, Janakpuri, New Delhi 110 058, India
Anil Khurana

²Central Council for Research in Homeopathy, 61-65, Institutional Area, Opp. D-Block, Janakpuri, New Delhi 110 058, India
Debadatta Nayak

²Central Council for Research in Homeopathy, 61-65, Institutional Area, Opp. D-Block, Janakpuri, New Delhi 110 058, India
Alok Kumar

²Central Council for Research in Homeopathy, 61-65, Institutional Area, Opp. D-Block, Janakpuri, New Delhi 110 058, India
Balasubramanian Karundevi

¹Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Sekkizhar Campus, Taramani, Chennai 600 113, India

Subject Editor:

Abstract

Background: Homeopathy is a holistic method of treatment that uses microdoses of natural substances originating from plants, minerals, or animal parts. Syzygium jambolanum and Cephalandra indica are used in homeopathy for treatment of type-2 diabetes. However, the molecular mechanisms responsible for such effects are not known.

Methods: Homeopathic preparations of S. jambolanum and C. indica in mother tincture, 6c and 30c were used to examine the molecular mechanism of antidiabetic effects in the skeletal muscle of rats with high fat and fructose-induced type-2 diabetes mellitus. After 30 days treatment, fasting blood glucose, serum insulin and insulin signaling molecules in the skeletal muscle (gastrocnemius) were measured.

Results: Diabetic rats showed a significant decrease in serum insulin and lipid profile as well as low levels of insulin receptor (IR), v-akt murine thymoma viral oncogene homolog (Akt), p-Akt^ser473 and glucose transporter-4 (GLUT4) protein expression (p < 0.05) with a significant increase in fasting blood glucose level (p < 0.05) compared to the control group. Treatment with homeopathic remedies significantly increased the serum insulin and expression of these proteins (p < 0.05) with a significant decrease in fasting blood glucose (p < 0.05) compared to diabetic rats.

Conclusions: In the present study homeopathic preparations of S. jambolanum and C. indica, including ultramolecular dilutions exhibit antidiabetic effects, improving insulin action through activation of insulin signaling molecules in skeletal muscle of type-2 diabetic rats.

Keywords

Insulin signaling - Homeopathic preparation - Syzygium jambolanum and Cephalandra indica - Gastrocnemius muscle

Full Text

References

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