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CC BY-NC-ND 4.0 · International Journal of Epilepsy 2017; 04(02): 159-166
DOI: 10.1016/j.ijep.2017.05.002
Research paper
Thieme Medical and Scientific Publishers Private Ltd.

Hydroalcoholic extract of Sargassum Oligocystum attenuates pentylenetetrazole-induced seizures by potentiating antioxidant activity in mice

Ali Movahed
a   Department of Biochemistry, Bushehr University of Medical Sciences, Bushehr, Iran
,
Mahbubeh Ghaderi
b   School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
,
Adel Daneshi
c   Department of Marine Toxicology, The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, Iran
,
Iraj Nabipour
c   Department of Marine Toxicology, The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, Iran
,
Mojtaba Keshavarz
d   Department of Pharmacology, Bushehr University of Medical Sciences, Bushehr, Iran
e   Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
› Author Affiliations
Further Information

Publication History

Received: 21 January 2017

Accepted: 15 May 2017

Publication Date:
06 May 2018 (online)

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Abstract

Objectives The aim of this study was to investigate the potential effects of Sargassum oligocystum extract on the pentylenetetrazole (PTZ) seizure and the contribution of antioxidant capacity of this alga to its antiepileptic effect.

Methods A dose of 100 mg/kg PTZ was used to induce the seizure in the male albino mice. Extract of Sargassum oligocystum in four doses (100, 200, 400 and 600 mg/kg), diazepam (5 mg/kg) and the vehicle were used 30 min before the injection of PTZ (n = 8). The onsets of clonic and tonic-clonic seizures, as well as the latency of death of animals, were recorded and the total antioxidant capacity (TAC), Superoxide dismutase (SOD) activity and catalase level were measured. Data were analyzed using one-way ANOVA or Kruskal-Wallis tests.

Results Sargassum oligocystum extract at the doses of 400 and 600 mg/kg significantly increased the latency of clonic and tonic-clonic seizures. Also, at the doses of 100, 200 and 400 mg/kg significantly increased the TAC. Moreover, Sargassum oligocystum at the doses of 200 and 400 mg/kg increased the SOD activity and at the doses of 400 and 600 mg/kg increased the catalase level in neural cells compared with the vehicle-treated group.

Conclusion Sargassum oligocystum extract inhibited PTZ-induced seizure. Attenuation of oxidative stress may partly be responsible for the anticonvulsant effects of this alga in the PTZ-induced seizures. Therefore, marine algae, especially Sargassum oligocystum, may be a valuable target to discover new antiepileptic drugs.

Keywords

Mice - Oxidative stress - Pentylenetetrazole - Sargassum oligocystum - Seizure
 

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