Int J Sports Med 2019; 40(03): 158-164
DOI: 10.1055/a-0828-8047
Physiology & Biochemistry
© Georg Thieme Verlag KG Stuttgart · New York

Supervised Short-term High-intensity Training on Plasma Irisin Concentrations in Type 2 Diabetic Patients

Tobias Dünnwald*
1   Department of Psychology and Medical Sciences, Institute for Sports Medicine, Alpine Medicine & Health Tourism (ISAG), Hall, Austria
Andreas Melmer*
2   Universitätsklinik für Diabetologie, Endokrinologie, Inselspital, Universitätsspital Bern, Bern, Switzerland
Hannes Gatterer
3   Institute of Mountain Emergency Medicine, EURAC Research, Bolzano, Italy
Karin Salzmann
4   Department of Internal Medicine I, Medical University of Innsbruck, Innsbruck, Austria
Christoph Ebenbichler
4   Department of Internal Medicine I, Medical University of Innsbruck, Innsbruck, Austria
Martin Burtscher
5   Sport Science, University of Innsbruck, Innsbruck, Austria
Wolfgang Schobersberger
6   Department of Psychology, Medical Sciences & Health Systems Management, University for Health Sciences, Medical Informatics and Technology, Hall in Tyrol, Austria
7   Tirol Kliniken GmbH Innsbruck, Institute for Sports Medicine, Alpine Medicine & Health Tourism (ISAG), Innsbruck, Austria
Wilhelm Grander
8   Department of Internal Medicine, Landeskrankenhaus Hall, Hall, Austria
› Author Affiliations
Further Information

Publication History

accepted 11 December 2018

Publication Date:
31 January 2019 (online)


Irisin is a myokine involved in adipocyte transformation. Its main beneficial effects arise from increased energy expenditure. Irisin production is particularly stimulated by physical exercise. The present study investigates the changes of plasma irisin in type 2 diabetic patients performing 2 different training modalities. Fourteen type 2 diabetic patients underwent 4 week of supervised high-intensity interval training (HIT; n=8) or continuous moderate-intensity training (CMT; n=6), with equivalent total amounts of work required. Plasma samples were collected in the resting state atbaseline and one day after the exercise intervention to analyse resting plasma irisin, blood lipids, blood glucose, hsCRP, Adiponectin, Leptin and TNF-α concentrations. In addition, body composition and VO2peak were determined Resting plasma irisin increased after HIT (p=0.049) and correlated significantly with plasma fasting glucose at follow-up (r=0.763; p=0.006). CMT did not significantly change the amount of plasma irisin, although follow-up values of plasma irisin correlated negatively with fat-free mass (r=−0.827, p=0.002) and with fasting plasma glucose (r = − 0.934, p=0.006). Plasma irisin was found to increase with higher training intensity, confirming the assumption that exercise intensity, in addition to the type of exercise, may play an important role in the stimulation of the irisin response.

* First and Second author contributed equally to this work.

  • References

  • 1 Al-Daghri NM, Alkharfy KM, Rahman S, Amer OE, Vinodson B, Sabico S, Piya MK, Harte AL, McTernan PG, Alokail MS, Chrousos GP. Irisin as a predictor of glucose metabolism in children: sexually dimorphic effects. Eur J Clin Invest 2014; 44: 119-124
  • 2 Archundia-Herrera C, Macias-Cervantes M, Ruiz-Munoz B, Vargas-Ortiz K, Kornhauser C, Perez-Vazquez V. Muscle irisin response to aerobic vs HIIT in overweight female adolescents. Diabetol Metab Syndr 2017; 9: 101
  • 3 Askari H, Rajani SF, Poorebrahim M, Haghi-Aminjan H, Raeis-Abdollahi E, Abdollahi M. A glance at the therapeutic potential of irisin against diseases involving inflammation, oxidative stress, and apoptosis: An introductory review. Pharmacol Res 2018; 129: 44-55
  • 4 Balady GJ, Arena R, Sietsema K, Myers J, Coke L, Fletcher GF, Forman D, Franklin B, Guazzi M, Gulati M, Keteyian SJ, Lavie CJ, Macko R, Mancini D, Milani RV. Clinician’s guide to cardiopulmonary exercise testing in adults: a scientific statement from the American Heart Association. Circulation 2010; 122: 191-225
  • 5 Bostrom P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, Rasbach KA, Bostrom EA, Choi JH, Long JZ, Kajimura S, Zingaretti MC, Vind BF, Tu H, Cinti S, Hojlund K, Gygi SP, Spiegelman BM. A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature 2012; 481: 463-468
  • 6 Chen N, Li Q, Liu J, Jia S. Irisin, an exercise-induced myokine as a metabolic regulator: an updated narrative review. Diabetes Metab Res Rev 2016; 32: 51-59
  • 7 Daskalopoulou SS, Cooke AB, Gomez YH, Mutter AF, Filippaios A, Mesfum ET, Mantzoros CS. Plasma irisin levels progressively increase in response to increasing exercise workloads in young, healthy, active subjects. Eur J Endocrinol 2014; 171: 343-352
  • 8 Eckardt K, Gorgens SW, Raschke S, Eckel J. Myokines in insulin resistance and type 2 diabetes. Diabetologia 2014; 57: 1087-1099
  • 9 Harriss DJ, Macsween A, Atkinson G. Standards for ethics in sport and exercise science research: 2018 Update. Int J Sports Med 2017; 38: 1126-1131
  • 10 Hecksteden A, Wegmann M, Steffen A, Kraushaar J, Morsch A, Ruppenthal S, Kaestner L, Meyer T. Irisin and exercise training in humans - results from a randomized controlled training trial. BMC Med 2013; 11: 235
  • 11 Howden EJ, Sarma S, Lawley JS, Opondo M, Cornwell W, Stoller D, Urey MA, Adams-Huet B, Levine BD. Reversing the cardiac effects of sedentary aging in middle age-a randomized controlled trial: Implications for heart failure prevention. Circulation 2018; 137: 1549-1560
  • 12 Huh JY, Panagiotou G, Mougios V, Brinkoetter M, Vamvini MT, Schneider BE, Mantzoros CS. FNDC5 and irisin in humans: I. Predictors of circulating concentrations in serum and plasma and II. mRNA expression and circulating concentrations in response to weight loss and exercise. Metabolism 2012; 61: 1725-1738
  • 13 Huh JY, Siopi A, Mougios V, Park KH, Mantzoros CS. Irisin in response to exercise in humans with and without metabolic syndrome. J Clin Endocrinol Metab 2015; 100: E453-E457
  • 14 Kraemer RR, Shockett P, Webb ND, Shah U, Castracane VD. A transient elevated irisin blood concentration in response to prolonged, moderate aerobic exercise in young men and women. Horm Metab Res 2014; 46: 150-154
  • 15 Kurdiova T, Balaz M, Vician M, Maderova D, Vlcek M, Valkovic L, Srbecky M, Imrich R, Kyselovicova O, Belan V, Jelok I, Wolfrum C, Klimes I, Krssak M, Zemkova E, Gasperikova D, Ukropec J, Ukropcova B. Effects of obesity, diabetes and exercise on Fndc5 gene expression and irisin release in human skeletal muscle and adipose tissue: in vivo and in vitro studies. J Physiol 2014; 592: 1091-1107
  • 16 Lee P, Linderman JD, Smith S, Brychta RJ, Wang J, Idelson C, Perron RM, Werner CD, Phan GQ, Kammula US, Kebebew E, Pacak K, Chen KY, Celi FS. Irisin and FGF21 are cold-induced endocrine activators of brown fat function in humans. Cell Metab 2014; 19: 302-309
  • 17 Li DJ, Huang F, Lu WJ, Jiang GJ, Deng YP, Shen FM. Metformin promotes irisin release from murine skeletal muscle independently of AMP-activated protein kinase activation. Acta Physiol (Oxf) 2015; 213: 711-721
  • 18 Liu JJ, Liu S, Wong MD, Tan CS, Tavintharan S, Sum CF, Lim SC. Relationship between circulating irisin, renal function and body composition in type 2 diabetes. J Diabetes Complications 2014; 28: 208-213
  • 19 Liu JJ, Wong MD, Toy WC, Tan CS, Liu S, Ng XW, Tavintharan S, Sum CF, Lim SC. Lower circulating irisin is associated with type 2 diabetes mellitus. J Diabetes Complications 2013; 27: 365-369
  • 20 Miyamoto-Mikami E, Sato K, Kurihara T, Hasegawa N, Fujie S, Fujita S, Sanada K, Hamaoka T, Tabata I, Iemitsu M. Endurance training-induced increase in circulating irisin levels is associated with reduction of abdominal visceral fat in middle-aged and older adults. Plos One 2015; 10: e0120354
  • 21 Moreno-Navarrete JM, Ortega F, Serrano M, Guerra E, Pardo G, Tinahones F, Ricart W, Fernandez-Real JM. Irisin is expressed and produced by human muscle and adipose tissue in association with obesity and insulin resistance. J Clin Endocrinol Metab 2013; 98: E769-E778
  • 22 Moreno M, Moreno-Navarrete JM, Serrano M, Ortega F, Delgado E, Sanchez-Ragnarsson C, Valdes S, Botas P, Ricart W, Fernandez-Real JM. Circulating irisin levels are positively associated with metabolic risk factors in sedentary subjects. Plos One 2015; 10: e0124100
  • 23 Norheim F, Langleite TM, Hjorth M, Holen T, Kielland A, Stadheim HK, Gulseth HL, Birkeland KI, Jensen J, Drevon CA. The effects of acute and chronic exercise on PGC-1alpha, irisin and browning of subcutaneous adipose tissue in humans. FEBS J 2014; 281: 739-749
  • 24 Park KH, Zaichenko L, Brinkoetter M, Thakkar B, Sahin-Efe A, Joung KE, Tsoukas MA, Geladari EV, Huh JY, Dincer F, Davis CR, Crowell JA, Mantzoros CS. Circulating irisin in relation to insulin resistance and the metabolic syndrome. J Clin Endocrinol Metab 2013; 98: 4899-4907
  • 25 Pedersen BK, Febbraio MA. Muscles, exercise and obesity: skeletal muscle as a secretory organ. Nat Rev Endocrinol 2012; 8: 457-465
  • 26 Pekkala S, Wiklund PK, Hulmi JJ, Ahtiainen JP, Horttanainen M, Pollanen E, Makela KA, Kainulainen H, Hakkinen K, Nyman K, Alen M, Herzig KH, Cheng S. Are skeletal muscle FNDC5 gene expression and irisin release regulated by exercise and related to health?. J Physiol 2013; 591: 5393-5400
  • 27 Qiu S, Bosnyak E, Treff G, Steinacker JM, Niess AM, Kruger K, Mooren FC, Zugel M, Schumann U. Acute exercise-induced irisin release in healthy adults: Associations with training status and exercise mode. Eur J Sport Sci 2018; 18: 1226-12331
  • 28 Qiu S, Cai X, Sun Z, Schumann U, Zugel M, Steinacker JM. Chronic exercise training and circulating irisin in adults: A meta-analysis. Sports Med 2015; 45: 1577-1588
  • 29 Roca-Rivada A, Castelao C, Senin LL, Landrove MO, Baltar J, Belen Crujeiras A, Seoane LM, Casanueva FF, Pardo M. FNDC5/irisin is not only a myokine but also an adipokine. Plos One 2013; 8: e60563
  • 30 Rognmo O, Hetland E, Helgerud J, Hoff J, Slordahl SA. High intensity aerobic interval exercise is superior to moderate intensity exercise for increasing aerobic capacity in patients with coronary artery disease. Eur J Cardiovasc Prev Rehabil 2004; 11: 216-222
  • 31 Safarimosavi S, Mohebbi H, Rohani H. High-intensity interval vs. continuous endurance training: Preventive effects on hormonal changes and physiological adaptations in prediabetes patients. J Strength Cond Res 2018; DOI: 10.1519/jsc.0000000000002709.
  • 32 Scalzo RL, Peltonen GL, Giordano GR, Binns SE, Klochak AL, Paris HL, Schweder MM, Szallar SE, Wood LM, Larson DG, Luckasen GJ, Hickey MS, Bell C. Regulators of human white adipose browning: evidence for sympathetic control and sexual dimorphic responses to sprint interval training. Plos One 2014; 9: e90696
  • 33 Shanaki M, Moradi N, Emamgholipour S, Fadaei R, Poustchi H. Lower circulating irisin is associated with nonalcoholic fatty liver disease and type 2 diabetes. Diabetes Metab Syndr 2017; 11 (Suppl. 01) S467-S472
  • 34 Shelbaya S, Abu Shady MM, Nasr MS, Bekhet MM, Mageed YA, Abbas M. Study of irisin hormone level in type 2 diabetic patients and patients with diabetic nephropathy. Curr Diabetes Rev 2018; 14: 481-486
  • 35 Shirvani H, Arabzadeh E. Metabolic cross-talk between skeletal muscle and adipose tissue in high-intensity interval training vs. moderate-intensity continuous training by regulation of PGC-1alpha. Eat Weight Disord 2018; DOI: 10.1007/s40519–018–0491–4.
  • 36 Shrestha P, Ghimire L. A review about the effect of life style modification on diabetes and quality of life. Global J Health Sci 2012; 4: 185-190
  • 37 Stengel A, Hofmann T, Goebel-Stengel M, Elbelt U, Kobelt P, Klapp BF. Circulating levels of irisin in patients with anorexia nervosa and different stages of obesity–correlation with body mass index. Peptides 2013; 39: 125-130
  • 38 Tsuchiya Y, Ando D, Goto K, Kiuchi M, Yamakita M, Koyama K. High-intensity exercise causes greater irisin response compared with low-intensity exercise under similar energy consumption. Tohoku J Exp Med 2014; 233: 135-140
  • 39 Tsuchiya Y, Ando D, Takamatsu K, Goto K. Resistance exercise induces a greater irisin response than endurance exercise. Metabolism 2015; 64: 1042-1050
  • 40 Winn NC, Grunewald ZI, Liu Y, Heden TD, Nyhoff LM, Kanaley JA. Plasma irisin modestly increases during moderate and high-intensity afternoon exercise in obese females. Plos One 2017; 12: e0170690
  • 41 Wu J, Bostrom P, Sparks LM, Ye L, Choi JH, Giang AH, Khandekar M, Virtanen KA, Nuutila P, Schaart G, Huang K, Tu H, van Marken Lichtenbelt WD, Hoeks J, Enerback S, Schrauwen P, Spiegelman BM. Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human. Cell 2012; 150: 366-376
  • 42 Zugel M, Qiu S, Laszlo R, Bosnyak E, Weigt C, Muller D, Diel P, Steinacker JM, Schumann U. The role of sex, adiposity, and gonadectomy in the regulation of irisin secretion. Endocrine 2016; 54: 101-110