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Int J Sports Med 2019; 40(14): 931-940
DOI: 10.1055/a-1015-0285
Genetics & Molecular Biology
© Georg Thieme Verlag KG Stuttgart · New York

MiR-214 Attenuates the Osteogenic Effects of Mechanical Loading on Osteoblasts

Yu Yuan
1   School of Physical Education and Sports Science, South China Normal University, Guangzhou, China
2   School of Kinesiology, Shanghai University of Sport, Shanghai, China
,
Jianmin Guo
2   School of Kinesiology, Shanghai University of Sport, Shanghai, China
,
Lingli Zhang
2   School of Kinesiology, Shanghai University of Sport, Shanghai, China
,
Xiaoyang Tong
2   School of Kinesiology, Shanghai University of Sport, Shanghai, China
,
Shihua Zhang
2   School of Kinesiology, Shanghai University of Sport, Shanghai, China
,
Xuchang Zhou
2   School of Kinesiology, Shanghai University of Sport, Shanghai, China
,
Miao Zhang
2   School of Kinesiology, Shanghai University of Sport, Shanghai, China
,
Xi Chen
3   School of Sports Science, Wenzhou Medical University, Wenzhou, Shanghai, China
,
Le Lei
2   School of Kinesiology, Shanghai University of Sport, Shanghai, China
,
Hui Li
2   School of Kinesiology, Shanghai University of Sport, Shanghai, China
,
Timon Cheng Yi Liu
4   Guangzhou Higher Education Mega Center, Laboratory of Laser Sports Medicine, South China Normal University, Guangzhou, China
,
Jiake Xu
5   School of Biomedical Sciences, University of Western Australia, Crawley, Australia
,
Jun Zou
2   School of Kinesiology, Shanghai University of Sport, Shanghai, China
› Author Affiliations Acknowledgements: The study is supported by funding from Shanghai Key Lab of Human Sport Competence Development and Maintenance (Shanghai University of sport) (NO. 11DZ2261100), the National Natural Science Foundation of China (NO. 81572242), China Postdoctoral Science Foundation funded project (NO.2018M640792, 2019T120739) and Research and development fund for young teachers (South China Normal University) (NO. 18KJ07).
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Publication History



accepted 03 September 2019

Publication Date:
22 October 2019 (online)

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Abstract

Exercise is an effective way to prevent osteoporosis, but its mechanism remains unclear. MicroRNAs (miRNAs) play an essential role in bone metabolism. Recently, mechanical loading was reported to induce changes in miRNA expression in osteoblasts. However, the role of miRNAs in bone under exercise and its underlining mechanisms of action still remain unknown. MiR-214 was reported to regulate the process of osteogenesis and is considered a biomarker of osteoporosis. In this study, we aimed to investigate whether exercise could induce changes in miRNA expression in bone and to study the effects of miR-214 on mechanical loading-induced osteogenesis in osteoblasts. The results showed that miR-214 was down-regulated in both tibia from C57BL/6 mice after exercise in vivo and in osteoblasts after mechanical strain in vitro. Mechanical strain could enhance the ALP activity, promote matrix mineralization, up-regulate the expression of osteogenic factors such as ATF4, Osterix, ALP and β-catenin, and down-regulate RANKL and RANK expression. Over-expression of miR-214 not only inhibited the expression of these osteogenic factors but also attenuated mechanical strain-enhanced osteogenesis in osteoblasts. Collectively, our results indicated that miR-214 could attenuate the osteogenic effects of mechanical loading on osteoblasts, suggesting that inhibition of miR-214 may be one of the ways in which exercise prevents osteoporosis.

Key words

exercise - mechanical strain - miR-214 - osteoblasts - osteoporosis
 

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