Lung Based Engineered Micro-Pancreas Sustains Human Beta Cell Survival and Functionality

Orit Goldman; Dmitry Puchinsky; Karina Durlacher; Rocio Sancho; Barbara Ludwig; Patrick Kugelmeier; Carolin Heller; Nikolai Kunicher; Stefan R. Bornstein; Avraham J. Treves

doi:10.1055/a-1041-3305

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 2019; 51(12): 805-811
DOI: 10.1055/a-1041-3305

Endocrine Research

Lung Based Engineered Micro-Pancreas Sustains Human Beta Cell Survival and Functionality

Orit Goldman

¹Betalin Therapeutics LTD, Jerusalem Bio-Park, Jerusalem, Israel

,

Dmitry Puchinsky

¹Betalin Therapeutics LTD, Jerusalem Bio-Park, Jerusalem, Israel

,

Karina Durlacher

¹Betalin Therapeutics LTD, Jerusalem Bio-Park, Jerusalem, Israel

,

Rocio Sancho

²Centre for Stem Cells and Regenerative Medicine, King’s College London, London, UK

³Department of Internal Medicine III, University Hospital Carl Gustav Carus at the Technische Universität Dresden, Dresden, Germany

,

Barbara Ludwig

³Department of Internal Medicine III, University Hospital Carl Gustav Carus at the Technische Universität Dresden, Dresden, Germany

⁴Paul Langerhans Institute Dresden of Helmholtz Centre Munich at University Hospital Carl Gustav Carus of Technische Universität Dresden and German Centre for Diabetes Research, Dresden, Germany

⁵Center for Regenerative Therapies, Technische Universität Dresden, Dresden, Germany

,

Patrick Kugelmeier

⁶Kugelmeiers Ltd, Erlenbach, Switzerland

,

Carolin Heller

³Department of Internal Medicine III, University Hospital Carl Gustav Carus at the Technische Universität Dresden, Dresden, Germany

⁴Paul Langerhans Institute Dresden of Helmholtz Centre Munich at University Hospital Carl Gustav Carus of Technische Universität Dresden and German Centre for Diabetes Research, Dresden, Germany

⁵Center for Regenerative Therapies, Technische Universität Dresden, Dresden, Germany

,

Nikolai Kunicher

¹Betalin Therapeutics LTD, Jerusalem Bio-Park, Jerusalem, Israel

,

Stefan R. Bornstein

³Department of Internal Medicine III, University Hospital Carl Gustav Carus at the Technische Universität Dresden, Dresden, Germany

⁴Paul Langerhans Institute Dresden of Helmholtz Centre Munich at University Hospital Carl Gustav Carus of Technische Universität Dresden and German Centre for Diabetes Research, Dresden, Germany

⁵Center for Regenerative Therapies, Technische Universität Dresden, Dresden, Germany

⁷Division of Diabetes & Nutritional Sciences, Faculty of Life Sciences & Medicine, King’s College London, London, UK

⁸Klinik für Endokrinologie, Diabetologie und Klinische Ernährung, University Hospital, Zürich, Switzerland

,

Avraham J. Treves

¹Betalin Therapeutics LTD, Jerusalem Bio-Park, Jerusalem, Israel

› Author Affiliations

Abstract

The whole world has been affected by a dramatically increasing prevalence of diabetes. Today, the etiology of both type 1 and type 2 diabetes is thought to revolve around the dysfunction of β-cells, the insulin producing cells of the body. Within the pharmaceutical industry, the evaluation of new drugs for diabetes treatment is mostly done using cell lines or rodent islets and depends solely on the assessment of static insulin secretion. However, the use of cell lines or rodent islets is limiting lack of similarity of the human islet cells, leading to a constrain of the predictive value regarding the clinical potential of newly developed drugs. To overcome this issue, we developed an Engineered Micro-Pancreas as a unique platform for drug discovery. The Engineered Micro Pancreas is composed of (i) an organ-derived micro-scaffold, specifically a decellularized porcine lung-derived micro-scaffold and (ii) cadaveric islets seeded thereon. The Engineered Micro Pancreas remained viable and maintained insulin secretion in vitro for up to three months. The quantities of insulin were comparable to those secreted by freshly isolated human islets and therefore hold the potential for real-time and metabolic physiology mimicking drug screening.

Key words

human islets - engineered micro pancreas - drug development - scaffold - micro-organ-matrix

Full Text

References

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