Int J Sports Med 2020; 41(09): 582-588
DOI: 10.1055/a-1145-3595
Physiology & Biochemistry

Regulation of Circulatory Muscle-specific MicroRNA during 8 km Run

Xin Yin
1   School of life sciences, Nanjing University, Nanjing, China
,
Shufang Cui
1   School of life sciences, Nanjing University, Nanjing, China
,
Xin Li
1   School of life sciences, Nanjing University, Nanjing, China
,
Wei Li
2   The Research Center of Military Exercise Science, The Army Engineering University of PLA, Nanjing, China
,
Qiu ju Lu
2   The Research Center of Military Exercise Science, The Army Engineering University of PLA, Nanjing, China
,
Xiao hong Jiang
1   School of life sciences, Nanjing University, Nanjing, China
,
Hui Wang
3   Department of Ultrasound Diagnosis, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
,
Xi Chen
1   School of life sciences, Nanjing University, Nanjing, China
,
Ji zheng Ma
2   The Research Center of Military Exercise Science, The Army Engineering University of PLA, Nanjing, China
› Author Affiliations
Funding: The authors acknowledge that this work was supported by the National Basic Research Program of China (2014CB542300), the National Natural Science Foundation of China (81101330, 31271378, 81250044), the Natural Science Foundation of Jiangsu Province (BK2012014) and the Research Special Fund for Public Welfare Industry of Health (201302018). This work was also supported by the Program for New Century Excellent Talents in University from the Ministry of Education of China (NCET-12–0261).

Abstract

Acute prolonged endurance running has been shown to alter muscle-specific circulating microRNA (miRNA) levels. Here, eighteen participants completed an 8 km run. We assessed the levels of hsa-miR-1–3p, -133a-3p, -133b, and -206 and their correlation with conventional biomarkers following exercise. Compared to before exercise (Pre), 8 km run significantly increased the lactate level immediately after exercise (0 h). Myoglobin (Mb) level increased at 0 h while creatine kinase (CK) level increased 24 h after exercise (24 h). The levels of creatine kinase MB isoenzyme (CK-MB) and cardiac troponin I (cTnI) were all elevated at 24 h and within the normal physiological range; The levels of hsa-miR-1–3p, -133a-3p, -133b significantly increased at 0 h but only hsa-miR-133a-3p still elevated at 24 h. Only hsa-miR-206 level decreased at 24 h; Additionally, the changes of hsa-miR-1–3p and hsa-miR-133a-3p were correlated with Mb at 24 h. These findings suggest that muscle-specific miRNA elevation in plasma is likely physiological and that these miRNA may be used as potential biomarkers for load monitoring in individuals.



Publication History

Received: 00 00 2020

Accepted: 30 April 2020

Article published online:
30 April 2020

© Georg Thieme Verlag KG
Stuttgart · New York

 
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