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Non-oxidative Energy Supply Correlates with Lactate Transport and Removal in Trained RowersFunding This study was funded by the French Ministry of Sport and INSEP.
This study aimed to test if the non-oxidative energy supply (estimated by the accumulated oxygen deficit) is associated with an index of muscle lactate accumulation during exercise, muscle monocarboxylate transporter content and the lactate removal ability during recovery in well-trained rowers. Seventeen rowers completed a 3-min all-out exercise on rowing ergometer to estimate the accumulated oxygen deficit. Blood lactate samples were collected during the subsequent passive recovery to assess individual blood lactate curves, which were fitted to the bi-exponential time function: La(t)= [La](0)+A1·(1–e–γ 1 t)+A2·(1–e–γ 2 t), where the velocity constants γ1 and γ2 (min–1) denote the lactate exchange and removal abilities during recovery, respectively. The accumulated oxygen deficit was correlated with the net amount of lactate released from the previously active muscles (r =0.58, P<0.05), the monocarboxylate transporters MCT1 and MCT4 (r=0.63, P<0.05) and γ2 (r=0.55, P<0.05). γ2 and the lactate release rate at exercise completion were negatively correlated with citrate synthase activity. These findings suggest that the capacity to supply non-oxidative energy during supramaximal rowing exercise is associated with muscle lactate accumulation and transport, as well as lactate removal ability.
Key wordsaccumulated oxygen deficit - monocarboxylate transporters - lactate exchange ability - mathematical model - rowing
† deceased May 11th 2016.
Received: 13 May 2020
Accepted: 13 May 2020
08 July 2020 (online)
Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany
- 1 Medbo JI, Mohn AC, Tabata I. et al. Anaerobic capacity determined by maximal accumulated O2 deficit. J Appl Physiol (1985) 1988; 64: 50-60. DOI: 10.1152/jappl.19188.8.131.52.
- 2 Bangsbo J. Quantification of anaerobic energy production during intense exercise. Med Sci Sports Exerc 1998; 30: 47-52 doi:10.1097/00005768-199801000-00007
- 3 Noordhof DA, de Koning JJ, Foster C. The maximal accumulated oxygen deficit method: a valid and reliable measure of anaerobic capacity?. Sports Med 2010; 40: 285-302 doi:10.2165/11530390-000000000-00000
- 4 Saltin B Anaerobic capacity: Past, present, and prospective. In: Taylor AW, Gollnick PD, Green HJ et al, Eds. Biochemistry of Exercise VII. Champaign, IL: Human Kinetics; 1990: 387–411
- 5 Marcinek DJ, Kushmerick MJ, Conley KE. Lactic acidosis in vivo: Testing the link between lactate generation and H+ accumulation in ischemic mouse muscle. J Appl Physiol (1985) 2010; 108: 1479-1486. doi:10.1152/japplphysiol.01189.2009
- 6 Juel C, Bangsbo J, Graham T. et al. Lactate and potassium fluxes from human skeletal muscle during and after intense, dynamic, knee extensor exercise. Acta Physiol Scand 1990; 140: 147-159. DOI: 10.1111/j.1748-1716.1990.tb08986.x.
- 7 Freund H, Lonsdorfer J, Oyono-Enguelle S. et al. Lactate exchange and removal abilities in sickle cell patients and in untrained and trained healthy humans. J Appl Physiol (1985) 1992; 73: 2580-2587. DOI: 10.1152/jappl.19184.108.40.20680.
- 8 Osnes JB, Hermansen L. Acid-base balance after maximal exercise of short duration. J Appl Physiol 1972; 32: 59-63. doi:10.1152/jappl.19220.127.116.11
- 9 Nielsen HB, Hein L, Svendsen LB. et al. Bicarbonate attenuates intracellular acidosis. Acta Anaesthesiol Scand 2002; 46: 579-584 DOI: 10.1034/j.1399-6576.2002.460516.x.
- 10 Spriet LL, Matsos CG, Peters SJ. et al. Effects of acidosis on rat muscle metabolism and performance during heavy exercise. Am J Physiol 1985; 248: C337-C347. DOI: 10.1152/ajpcell.1985.248.3.C337.
- 11 Hollidge-Horvat MG, Parolin ML, Wong D. et al. Effect of induced metabolic acidosis on human skeletal muscle metabolism during exercise. Am J Physiol 1999; 277: E647-E658. DOI: 10.1152/ajpendo.1999.277.4.E647.
- 12 Costa Leite T, Da Silva D, Guimaraes Coelho R. et al. Lactate favours the dissociation of skeletal muscle 6-phosphofructo-1-kinase tetramers down-regulating the enzyme and muscle glycolysis. Biochem J 2007; 408: 123-130. DOI: 10.1042/BJ20070687.
- 13 Allen D, Westerblad H. Physiology. Lactic acid--the latest performance-enhancing drug. Science 2004; 305: 1112-1113 doi:10.1126/science.1103078
- 14 Pedersen TH, Nielsen OB, Lamb GD. et al. Intracellular acidosis enhances the excitability of working muscle. Science 2004; 305: 1144-1147. DOI: 10.1126/science.1101141.
- 15 Juel C. Regulation of pH in human skeletal muscle: adaptations to physical activity. Acta Physiol (Oxf) 2008; 193: 17-24 doi:10.1111/j.1748-1716.2008.01840.x
- 16 Juel C. Lactate-proton cotransport in skeletal muscle. Physiol Rev 1997; 77: 321-358. doi:10.1152/physrev.1918.104.22.1681
- 17 Bergman BC, Wolfel EE, Butterfield GE. et al. Active muscle and whole body lactate kinetics after endurance training in men. J Appl Physiol (1985) 1999; 87: 1684-1696. DOI: 10.1152/jappl.1922.214.171.1244.
- 18 Messonnier LA, Emhoff CA, Fattor JA. et al. Lactate kinetics at the lactate threshold in trained and untrained men. J Appl Physiol (1985) 2013; 114: 1593-1602. DOI: 10.1152/japplphysiol.00043.2013.
- 19 Messonnier L, Freund H, Bourdin M. et al. Lactate exchange and removal abilities in rowing performance. Med Sci Sports Exerc 1997; 29: 396-401 DOI: 10.1097/00005768-199703000-00016.
- 20 Messonnier L, Freund H, Denis C. et al. Time to exhaustion at VO(2)max is related to the lactate exchange and removal abilities. Int J Sports Med 2002; 23: 433-438 DOI: 10.1055/s-2002-33740.
- 21 Brooks GA. Lactate shuttle -- between but not within cells?. J Physiol 2002; 541: 333-334. doi:10.1113/jphysiol.2002.023705
- 22 Bangsbo J, Michalsik L, Petersen A. Accumulated O2 deficit during intense exercise and muscle characteristics of elite athletes. Int J Sports Med 1993; 14: 207-213. doi:10.1055/s-2007-1021165
- 23 Maciejewski H, Bourdin M, Lacour JR. et al. Lactate accumulation in response to supramaximal exercise in rowers. Scand J Med Sci Sports 2013; 23: 585-592 DOI: 10.1111/j.1600-0838.2011.01423.x.
- 24 Nielsen HB. pH after competitive rowing: the lower physiological range?. Acta Physiol Scand 1999; 165: 113-114. doi:10.1046/j.1365-201x.1999.00485.x
- 25 Harriss DJ, MacSween A, Atkinson G. Ethical standards in sport and exercise science research: 2020 update. Int J Sports Med 2019; 40: 813-817. doi:10.1055/a-1015-3123
- 26 Freund H, Zouloumian P. Lactate after exercise in man: I. Evolution kinetics in arterial blood. Eur J Appl Physiol Occup Physiol 1981; 46: 121-133. doi:10.1007/bf00428865
- 27 Freund H, Zouloumian P. Lactate after exercise in man: IV. Physiological observations and model predictions. Eur J Appl Physiol Occup Physiol 1981; 46: 161-176 doi:10.1007/bf00428868
- 28 Bret C, Lacour JR, Bourdin M. et al. Differences in lactate exchange and removal abilities between high-level African and Caucasian 400-m track runners. Eur J Appl Physiol 2013; 113: 1489-1498. DOI: 10.1007/s00421-012-2573-8.
- 29 Brooke MH, Kaiser KK. Muscle fiber types: How many and what kind?. Arch Neurol 1970; 23: 369-379. doi:10.1001/archneur.1970.00480280083010
- 30 Maciejewski H, Bourdin M, Feasson L. et al. Muscle MCT4 content is correlated with the lactate removal ability during recovery following all-out supramaximal exercise in highly-trained rowers. Front Physiol 2016; 7: 223. DOI: 10.3389/fphys.2016.00223.
- 31 Zoladz JA, Duda K, Majerczak J. VO2/power output relationship and the slow component of oxygen uptake kinetics during cycling at different pedaling rates: Relationship to venous lactate accumulation and blood acid-base balance. Physiol Res 1998; 47: 427-438
- 32 Bangsbo J, Gollnick PD, Graham TE. et al. Anaerobic energy production and O2 deficit-debt relationship during exhaustive exercise in humans. J Physiol 1990; 422: 539-559. DOI: 10.1113/jphysiol.1990.sp018000.
- 33 Majerczak J, Korostynski M, Nieckarz Z. et al. Endurance training decreases the non-linearity in the oxygen uptake-power output relationship in humans. Exp Physiol 2012; 97: 386-399 DOI: 10.1113/expphysiol.2011.062992.
- 34 Zoladz JA, Majerczak J, Grassi B. et al. Mechanisms of attenuation of pulmonary V̇O2 slow component in humans after prolonged endurance training. PLoS One 2016; 11: e0154135. DOI: 10.1371/journal.pone.0154135.
- 35 Freund H, Zouloumian P, Oyono-Enguéllé S. et al. Lactate kinetics after maximal exercise in man. Med Sport Sci 1984; 17: 9-24
- 36 Sahlin K, Harris RC, Nylind B. et al. Lactate content and pH in muscle obtained after dynamic exercise. Pflugers Arch 1976; 367: 143-149. DOI: 10.1007/bf00585150.
- 37 Parkhouse WS, McKenzie DC, Hochachka PW. et al. Buffering capacity of deproteinized human vastus lateralis muscle. J Appl Physiol (1985) 1985; 58: 14-17 DOI: 10.1152/jappl.19126.96.36.199.
- 38 Dubouchaud H, Butterfield GE, Wolfel EE. et al. Endurance training, expression, and physiology of LDH, MCT1, and MCT4 in human skeletal muscle. Am J Physiol Endocrinol Metab 2000; 278: E571-E579. DOI: 10.1152/ajpendo.2000.278.4.E571.
- 39 Manning Fox JE, Meredith D, Halestrap AP. Characterisation of human monocarboxylate transporter 4 substantiates its role in lactic acid efflux from skeletal muscle. J Physiol 2000; 529 Pt 2 285-293. DOI: 10.1111/j.1469-7793.2000.00285.x.
- 40 Chatel B, Bendahan D, Hourde C. et al. Role of MCT1 and CAII in skeletal muscle pH homeostasis, energetics, and function: in vivo insights from MCT1 haploinsufficient mice. FASEB J 2017; 31: 2562-2575. DOI: 10.1096/fj.201601259R.
- 41 Dimmer KS, Friedrich B, Lang F. et al. The low-affinity monocarboxylate transporter MCT4 is adapted to the export of lactate in highly glycolytic cells. Biochem J 2000; 350 Pt 1: 219-227
- 42 Freund H, Oyono-Enguelle S, Heitz A. et al. Work rate-dependent lactate kinetics after exercise in humans. J Appl Physiol (1985) 1986; 61: 932-939 DOI: 10.1152/jappl.19188.8.131.522.
- 43 Emhoff CA, Messonnier LA, Horning MA. et al. Direct and indirect lactate oxidation in trained and untrained men. J Appl Physiol (1985) 2013; 115: 829-838. DOI: 10.1152/japplphysiol.00538.2013.
- 44 Thomas C, Perrey S, Lambert K. et al. Monocarboxylate transporters, blood lactate removal after supramaximal exercise, and fatigue indexes in humans. J Appl Physiol (1985) 2005; 98: 804-809. DOI: 10.1152/japplphysiol.01057.2004.
- 45 Van Hall G, Calbet JA, Sondergaard H. et al. Similar carbohydrate but enhanced lactate utilization during exercise after 9 wk of acclimatization to 5,620 m. Am J Physiol Endocrinol Metab 2002; 283: E1203-E1213. DOI: 10.1152/ajpendo.00134.2001.
- 46 Steinacker JM. Physiological aspects of training in rowing. Int J Sports Med 1993; 14 Suppl 1: S3-S10
- 47 Volianitis S, Secher NH, Quistorff B. Elevated arterial lactate delays recovery of intracellular muscle pH after exercise. Eur J Appl Physiol 2018; 118: 2429-2434. doi:10.1007/s00421-018-3969-x