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CC BY-NC-ND 4.0 · Thromb Haemost 2023; 123(07): 669-678
DOI: 10.1055/a-2075-8204
Coagulation and Fibrinolysis

Cryofibrinogen—Characteristics and Association with Cryoglobulin: A Retrospective Study Out of a Series of 1,712 Samples over 7 Years

Marie N. Sarda-Kolopp 
1   Immunology Lab, Hospices Civils de Lyon, Pierre Bénite, Auvergne-Rhône-Alpes, France
2   Immunogenomics and Inflammation Research Unit, Université Lyon 1 Faculté de Médecine Lyon-Est, Lyon, Auvergne-Rhône-Alpes, France
,
Pierre Miossec
2   Immunogenomics and Inflammation Research Unit, Université Lyon 1 Faculté de Médecine Lyon-Est, Lyon, Auvergne-Rhône-Alpes, France
3   Clinical Immunology Unit, Department of Immunology and Rheumatology, Hospices Civils de Lyon, Lyon, Auvergne-Rhône-Alpes, France
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Abstract

Objective Cryofibrinogens (CFs) and cryoglobulins (CGs) are cryoproteins responsible for obstructive vasculopathy and vasculitis. The aim of this study was to compare the characteristics of CF and CG, and to define the conditions of their association.

Methods and Results This retrospective study was conducted at the Lyon University Hospitals, and included patients with at least one sample tested for CF and/or CG between September 2013 and April 2021. Serum and plasma samples were analyzed in very strict conditions of temperature. After cold precipitation, CF and CG were characterized and quantified in the cryoprecipitates. CRP and plasma fibrinogen levels were also investigated. Over this 7-year period, 1,712 samples for CF detection and 25,650 samples for CG detection were sent to the laboratory. Simultaneous testing of CF and CG was performed in 1,453/1,712 samples (85%). CF was less often positive than CG (8.3 vs. 13.5%, p < 0.0001). In positive CF samples, CG was associated in 28.9% of cases. In CF, fibrinogen was associated with fibronectin in 98/142 (69%) samples, especially in highly concentrated CF. CF concentration was independent of C-reactive protein and plasma fibrinogen concentrations.

Conclusion The simultaneous detection of CF and CG is essential for the diagnosis of vasculitis or thromboembolic events and their treatment.

Keywords

cryofibrinogens - cryoglobulins - inflammatory - vasculitis

Authors' Contribution

Conceptualization: M.N.S.K. and P.M.; Methodology: M.N.S.K.; Validation: P.M.; Formal analysis: M.N.S.K. and P.M.; Resources: M.N.S.K.; Writing—original draft preparation: M.N.S.K.; Writing—review and editing: M.N.S.K.




Publication History

Received: 28 June 2022

Accepted: 01 March 2023

Accepted Manuscript online:
18 April 2023

Article published online:
23 May 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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