Shedding of feline panleukopenia virus DNA in cats in breeding catteries

Abstract

Objectives

The present study’s aims were to investigate fecal panleukopenia virus (FPV) DNA shedding in cats from breeding catteries and to identify factors associated with shedding, including the presence of diarrhea.

Methods

239 cats out of 40 catteries were included. Information on catteries and cats were obtained by a breeders’ questionnaire. Fecal samples of the cats were classified with the Purina fecal score and analyzed for FPV DNA by quantitative polymerase chain reaction (qPCR). Other gastrointestinal microorganisms were assessed by qPCR/RT-qPCR and fecal flotation. Information on the catteries, cats, and results of fecal sample analyses were statistically analyzed to determine factors that were associated with fecal FPV DNA shedding by Fisher’s exact test; p-values <0.05 were considered significant.

Results

Six of 239 cats (2.5%) shed FPV DNA with feces. Cycle threshold values of qPCR ranged from 24–37. All FPV DNA-positive cats were coinfected (with either Clostridium spp., Cryptosporidium spp., Giardia spp. and/or feline coronavirus). Fecal consistency of 3/6 FPV DNA-positive samples was diarrheic, and fecal FPV DNA shedding was significantly associated with diarrheic feces (p= 0.015; OR: 9.9, CI_95%: 1.2–78.7). No other factors significantly influenced FPV DNA shedding.

Conclusions and relevance

Fecal FPV DNA shedding was rarely observed among healthy cats from breeding catteries but maintaining proper vaccination programs against panleukopenia is crucial. Since FPV DNA shedding was linked to a higher likelihood of diarrheic feces, mild diarrhea in otherwise healthy cats should not be neglected and raise awareness about a potential risk of FPV within the cattery or other multi-cat environments.

Zusammenfassung

Ziele

Ziel der Studie war es herauszufinden, ob Katzen aus Katzenzuchten feline Panleukopenievirus- (FPV-) DNA mit dem Kot ausscheiden und mögliche Risikofaktoren (einschließlich Durchfall) zu ermitteln, die mit einer Virusausscheidung im Zusammenhang stehen könnten.

Material und Methoden

In die Studie wurden 239 Katzen aus 40 Katzenzuchten eingeschlossen. Informationen zu den Zuchten und Katzen wurden mit Hilfe von Fragebögen ermittelt, die von den Züchtern ausgefüllt wurden. Die Kotproben der Katzen wurden mit dem Purina-Kot-Score klassifiziert und mittels quantitativer Polymerase-Kettenreaktion (qPCR) auf FPV-DNA untersucht. Untersuchungen auf weitere gastrointestinale Mikroorganismen wurden mittels qPCR/RT-qPCR und Kotflotation durchgeführt. Für die statistische Auswertung der Faktoren, die mit einer fäkalen FPV-DNA-Ausscheidung in Verbindung stehen, wurden Informationen zu Zuchten, Katzen und die Ergebnisse der Analyse der Kotproben berücksichtigt (exakter Test nach Fisher); p-Werte <0,05 galten als signifikant.

Ergebnisse

6 von 239 Katzen (2,5%) schieden FPV-DNA mit dem Kot aus. Die mittels qPCR gemessenen Ct-Werte (cycle threshold) lagen zwischen 24–37. In allen 6 Kotproben wurden Koinfektionen (mit entweder Clostridium spp., Cryptosporidium spp., Giardia spp. und/oder felinem Coronavirus) nachgewiesen. Diarrhö lag bei 3/6 FPV-DNA-positiven Proben vor und war signifikant mit der fäkalen FPV-DNA-Ausscheidung assoziiert (p= 0,015; OR: 9,9, CI_95%: 1,2–78,7). Andere Faktoren hatten keinen signifikanten Einfluss auf die Ausscheidung von FPV-DNA.

Schlussfolgerung

Nur eine kleine Anzahl gesunder Katzen aus Katzenzuchten schied FPV-DNA mit dem Kot aus; ungeachtet dessen sind adäquate Impfungen gegen Panleukopenie essenziell. Da der Nachweis von FPV-DNA mit Diarrhö in Zusammenhang stand, sollte eine weiche Kotkonsistenz bei ansonsten gesunden Katzen nicht vernachlässigt werden und auf ein mögliches FPV-Risiko in Zuchten oder anderen Mehrkatzenhaushalten aufmerksam machen.

Keywords

FPV - parvovirus - diarrhea - quantitative polymerase chain reaction (qPCR) - fecal samples - multi-cat household

Schlüsselwörter

FPV - Parvovirus - Durchfall - quantitative Polymerase-Kettenreaktion (qPCR) - Kotproben - Mehrkatzenhaushalt

Publikationsverlauf

Eingereicht: 15. April 2025

Angenommen: 02. September 2025

Artikel online veröffentlicht:
15. Oktober 2025

• References

• 1 Rehme T, Hartmann K, Truyen U. et al. Feline panleukopenia outbreaks and risk factors in cats in animal shelters. Viruses 2022; 14: 1248
• 2 Bergmann M, Schwertler S, Speck S. et al. Faecal shedding of parvovirus deoxyribonucleic acid following modified live feline panleucopenia virus vaccination in healthy cats. Vet Rec 2019; 185: 83
• 3 Clegg SR, Coyne KP, Dawson S. et al. Canine parvovirus in asymptomatic feline carriers. Vet Microbiol 2012; 157: 78-85
• 4 Miyazawa T, Ikeda Y, Nakamura K. et al. Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam. Microbiol Immunol 1999; 43: 609-612
• 5 Johnson RH. Feline panleucopaenia virus: III.-Some properties compared to a feline herpes virus. Res Vet Sci 1966; 7: 112-115
• 6 Johnson RH. Feline panleucopaenia. Vet Rec 1969; 84: 338-340
• 7 Lamm CG, Rezabek GB. Parvovirus infection in domestic companion animals. Vet Clin North Am Small Anim Pract 2008; 38: 837-850 viii-ix
• 8 Uttenthal A, Lund E, Hansen M. Mink enteritis parvovirus. Stability of virus kept under outdoor conditions. APMIS 1999; 107: 353-358
• 9 Greene CE. Feline Parvovirus Infection; Feline Enteric Viral Infections. In: Greene CE, ed. Infectious Diseases of the Dog and Cat. 4th revised ed. Missouri: Saunders Elsevier; 2012: 80-90
• 10 Kruse BD, Unterer S, Horlacher K. et al. Prognostic factors in cats with feline panleukopenia. J Vet Intern Med 2010; 24: 1271-1276
• 11 Tuzio H. Feline Panleukopenia. In: Miller LA and KF H, eds. Infectious Disease Management in Animal Shelters. 1st ed. Iowa: Wiley-Blackwell; 2009: 183-196
• 12 Barrs VR. Feline Panleukopenia: A re-emergent disease. Vet Clin North Am Small Anim Pract 2019; 49: 651-670
• 13 Barrs VR, Brailey J, Allison JB. et al. Re-emergence of feline panleukopenia in Australia. 27th ECVIM-CA Congress. St. Julian&apos;s Malta. 2017 570.
• 14 Litster A, Benjanirut C. Case series of feline panleukopenia virus in an animal shelter. J Feline Med Surg 2014; 16: 346-353
• 15 Porporato F, Horzinek MC, Hofmann-Lehmann R. et al. Survival estimates and outcome predictors for shelter cats with feline panleukopenia virus infection. J Am Vet Med Assoc 2018; 253: 188-195
• 16 Van Brussel K, Carrai M, Lin C. et al. Distinct lineages of feline parvovirus associated with epizootic outbreaks in Australia, New Zealand and the United Arab Emirates. Viruses 2019; 11: 1155
• 17 Byrne P, Beatty JA, Šlapeta J. et al. Shelter-housed cats show no evidence of faecal shedding of canine parvovirus DNA. Vet J 2018; 239: 54-58
• 18 Carrai M, Decaro N, Van Brussel K. et al. Canine parvovirus is shed infrequently by cats without diarrhoea in multi-cat environments. Vet Microbiol 2021; 261: 109204
• 19 Leutenegger CM, Liu H, Pedersen NC. Cross-species transmission of canine parvovirus 2 to healthy shelter cats. J Vet Intern Med. 2015 1204.
• 20 Whitby BJ, Clegg SR, Wheeler VJ. et al. A pilot study to determine the potential for cross-species transmission of canine parvoviruses. 53rd BSAVA Annual Congress. Birmingham United Kingdome. 2010
• 21 Sabshin SJ, Levy JK, Tupler T. et al. Enteropathogens identified in cats entering a Florida animal shelter with normal feces or diarrhea. J Am Vet Med Assoc 2012; 241: 331-337
• 22 Addie DD, Toth S, Thompson H. et al. Detection of feline parvovirus in dying pedigree kittens. Vet Rec 1998; 142: 353-356
• 23 Ström Holst B, Frössling J. The Swedish breeding cat: population description, infectious diseases and reproductive performance evaluated by a questionnaire. J Feline Med Surg 2009; 11: 793-802
• 24 Felten S, Klein-Richers U, Unterer S. et al. Role of feline coronavirus as contributor to diarrhea in cats from breeding catteries. Viruses 2022; 14: 858
• 25 Klein-Richers U, Hartmann K, Hofmann-Lehmann R. et al. Prevalence of feline coronavirus shedding in German catteries and associated risk factors. Viruses 2020; 12: 1000
• 26 Felten S, Klein-Richers U, Unterer S. et al. Patterns of feline coronavirus shedding and associated factors in cats from breeding catteries. Viruses 2023; 15: 1279
• 27 Polak KC, Levy JK, Crawford PC. et al. Infectious diseases in large-scale cat hoarding investigations. Vet J 2014; 201: 189-195
• 28 Clopper CJ, Pearson ES. The use of confidence or fiducial limits illustrated in the case of the binomial. Biometrika. 1934; 26: 404-413
• 29 Wisconsin Veterinary Diagnostic Laboratory. University Wisconsin-Madison https://www.wvdl.wisc.edu/wp-content/uploads/2013/01/WVDL.Info_.PCR_Ct_Values1.pdf last accessed on 19th June 2024.
• 30 Pacini MI, Forzan M, Franzo G. et al. Feline Parvovirus Lethal Outbreak in a Group of Adult Cohabiting Domestic Cats. Pathogens 2023; 12: 822
• 31 Sharp NJ, Davis BJ, Guy JS. et al. Hydranencephaly and cerebellar hypoplasia in two kittens attributed to intrauterine parvovirus infection. J Comp Pathol 1999; 121: 39-53
• 32 Goericke-Pesch S, Packeiser EM. Reproductive management in catteries: optimising health and wellbeing through veterinarian-breeder collaboration. J Feline Med Surg 2022; 24: 881-904
• 33 Squires RA, Crawford C, Marcondes M. et al. 2024 guidelines for the vaccination of dogs and cats – compiled by the Vaccination Guidelines Group (VGG) of the World Small Animal Veterinary Association (WSAVA). J Small Anim Pract 2024; 65: 277-316
• 34 Jakel V, Cussler K, Hanschmann KM. et al. Vaccination against Feline Panleukopenia: implications from a field study in kittens. BMC Vet Res 2012; 8: 62
• 35 Van Brussel K, Wang X, Shi M. et al. The enteric virome of cats with feline panleukopenia differs in abundance and diversity from healthy cats. Transbound Emerg Dis 2022; 69: e2952-e2966