Horm Metab Res 2009; 41(3): 197-201
DOI: 10.1055/s-0028-1087206
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Cardiac Hormones Activate ERK 1/2 Kinases in Human Fibroblasts

Y. Sun 1 , E. J. Eichelbaum 1 , H. Wang 1 , D. L. Vesely 1
  • 1Departments of Internal Medicine, Molecular Pharmacology and Physiology James A. Haley Veterans Medical Center and University of South Florida Cardiac Hormone Center, Tampa, Florida, USA
Further Information

Publication History

received 19.05.2008

accepted 07.08.2008

Publication Date:
14 October 2008 (online)

Abstract

Two cardiac hormones, vessel dilator and kaliuretic peptide, localize to fibroblasts with immunohistochemistry. Vessel dilator and kaliuretic peptide were investigated in dose-response and time-sequenced experiments for their cell signaling of extracellular signal–regulated kinases 1/2 in human fibroblasts to test the hypothesis that these two cardiovascular hormones contribute to fibroblast proliferation by activating extracellular signal–regulated kinases 1/2. Vessel dilator at 10 pM (physiological range) enhanced the phosphorylation of extracellular signal–regulated kinases 1/2 by 188±9% (p<0.001) in 10 min and, maximally, by 200±10% in 15 min (p<0.001). Vessel dilator at 10 nM enhanced the phosphorylation of extracellular signal–regulated kinases 1/2 by 107±5% (p<0.01) in 10 min. Kaliuretic peptide at 10 pM enhanced the activation of extracellular signal–regulated kinases 1/2 by 389±19% in 10 min (p<0.001). Kaliuretic peptide at 10 nM enhanced the phosphorylation of extracellular signal–regulated kinases 1/2 by 82±4% (p<0.01). Our results show that both cardiac hormones activate extracellular signal–regulated kinases 1/2 in human fibroblasts, suggesting that they may have a role in enhancing fibroblast proliferation.

References

Correspondence

D. L. Vesely, MD, PhD 

Professor of Medicine,

Molecular Pharmacology & Physiology

Chief of Endocrinology

Diabetes, and Metabolism

J. A. Haley Veterans Medical Center-151

13000 Bruce B. Downs Blvd.

Tampa

33612 Florida

USA

Phone: +1/813/972 76 24

Fax: +1/813/972 76 23

Email: [email protected]