Horm Metab Res 2009; 41(1): 1-4
DOI: 10.1055/s-0028-1087209
Innovative Methods

© Georg Thieme Verlag KG Stuttgart · New York

Noninvasive Monitoring of Changes in Pancreatic Beta-cell Mass by Bioluminescent Imaging in MIP-luc Transgenic Mice

S.-Y. Park 1 , G. I. Bell 1
  • 1Departments of Medicine and Human Genetics, The University of Chicago, Chicago, Illinois, USA
Further Information

Publication History

received 04.04.2008

accepted 30.07.2008

Publication Date:
23 October 2008 (online)

Abstract

We have generated a transgenic mouse model (MIP-luc) that allows real-time imaging of insulin-secreting pancreatic beta cells in living mice. The beta cells of MIP-luc transgenic mice emit a light signal that can be visualized externally by bioluminescent imaging using specialized equipment. In order to determine whether the intensity of the bioluminescent signal accurately reflects changes in beta-cell mass rather than simply transcriptional modulation of the mouse insulin I promoter–luciferase transgene, we examined the correlation between the bioluminescent signal and the beta-cell mass in MIP-luc mice fed a regular or high-fat Western diet. Male MIP-luc mice were fed a standard rodent diet (5% of calories from fat) or a high-fat Western diet (42% from fat) beginning at 4 weeks of age. The bioluminescent signal and beta-cell mass were measured after 6 and 10 weeks on each diet. The body weight, beta-cell mass, and bioluminescent signal increased with age and increased further in mice fed a high-fat diet. There was a statistically significant correlation between beta-cell mass and bioluminescent signal (r2=0.660, p=0.00137). Thus, in vivo bioluminescent imaging can be used to noninvasively monitor changes in beta-cell mass in living MIP-luc mice, and it complements other approaches for monitoring beta-cell mass in states of insulin resistance, obesity, and diabetes.

References

Correspondence

S.-Y. Park

Department of Medicine

The University of Chicago

5841 S. Maryland Avenue

MC1027

Chicago

60637 Illinois

USA

Phone: +1/773/702 91 16

Fax: +1/773/702 92 37

Email: [email protected]