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Horm Metab Res 1979; 11(8): 459-463
DOI: 10.1055/s-0028-1092761
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© Georg Thieme Verlag KG Stuttgart · New York

Immunogenicity and Bioactivity of Glucagon, Modified at Methionine-27

W. J. Nooijen , H. J. Kempen
  • Gaubius Institute, Health Research Organization, TNO, Leiden, The Netherlands
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Publication Date:
17 December 2008 (online)

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Abstract

Porcine glucagon was modified at methionine-27 by methylation or oxidation. Antisera against the glucagon derivatives were obtained. One of these antisera showed a high affinity for glucagon, with no cross-reactivity with gut-GLI 1.

Biological activities of these derivatives were assessed on rat hepatocytes. Both derivatives had the same maximal glucose mobilising activity as native glucagon, but a decreased potency suggesting a crucial role of methionine in the binding of glucagon to its hepatic receptor.

Key words

Porcine Glucagon - Glucagon Derivative (Methionine-27) - Immunogenicity - Bioactivity