Kidneys from normal, diabetic-nonketotic and ketotic Chinese hamsters were homogenized,
fractionated and assayed for β-glucosidase and β-galactosidase activities. The kidneys
of the ketotic animals were enlarged but the protein content in each subcellular fraction
was similar in all three groups of animals. β-Glucosidase was found chiefly in the
soluble fraction and no difference was observed in these animals. β-Galactosidase
was distributed in both cytoplasmic and particulate fractions; difference in the specific
activity of β-galactosidase between control and ketotic animals was found in nuclear,
lysosomalmitochondrial, microsomal and soluble fractions.
β-Gaiactosidase-β-glucosidase - Kidney - Ketotic - Diabetic
