Horm Metab Res 1972; 4(4): 232-234
DOI: 10.1055/s-0028-1094055
Originals

© Georg Thieme Verlag KG Stuttgart · New York

Proinsulin Content of Adult and Fetal Pancreatic Tissue Extracted after Rapid Freezing in Situ[*]

D. H. Lockwood [**] , R. I. Misbin [***]
  • The Clayton Laboratories, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Further Information

Publication History

Publication Date:
07 January 2009 (online)

Abstract

The proinsulin and insulin content of fetal and adult porcine pancreas and adult rabbit pancreas were determined after the tissues were rapidly frozen in situ to prevent protein degradation. Proinsulin was found to constitute 2-3% of the total immunoreactive insulin. The cross-reactivity of porcine proinsulin to porcine insulin antibody was 33% that of insulin, so that the proinsulin content of porcine pancreas on a molar basis is approximately 7%. These results demonstrate that proinsulin does not represent a major storage form of insulin in either adult or fetal pancreas.

1 Supported by U.S. Public Health Service Research Grant AM- 13562

1 Supported by U.S. Public Health Service Research Grant AM- 13562

2 Recipient of U.S. Public Health Service Research Career Development Award AM - 42579

3 New Address: Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02115 (USA)