Horm Metab Res 2009; 41(4): 281-286
DOI: 10.1055/s-0028-1102914
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Visfatin Stimulates Production of Monocyte Chemotactic Protein-1 and Interleukin-6 in Human Vein Umbilical Endothelial Cells

S. W. Liu 1 , S. B. Qiao 1 , J. S. Yuan 1 , D. Q. Liu 2
  • 1Department of Cardiology, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
  • 2Department of Central Laboratory, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
Further Information

Publication History

received 01.07.2008

accepted 14.10.2008

Publication Date:
13 November 2008 (online)

Abstract

Monocyte chemotactic protein-1 and interleukin-6 are important inflammatory cytokines, which have close relationships with atherosclerosis. Visfatin is a novel adipokine involved in regulation of inflammatory cytokines, however, associations of visfatin with cytokines (MCP-1, IL-6) in human umbilical vein endothelial cells are unclear. The aim of this study was to determine whether visfatin has effects on the expression of MCP-1 and IL-6 in human umbilical vein endothelial cells. Enzyme-linked immunosorbent assay were used for measuring MCP-1 and IL-6 production in human umbilical vein endothelial cells. Real-time quantitative reverse-transcription polymerase chain reaction was used for determining MCP-1 and IL-6 mRNA expression. For the pathway determination following inhibitors were used: wortmannin [phosphatiylinositol 3-kinase (PI3K)], SB203580 [p38 mitogen-activated protein kinase (MAPK)], PD98059 [extracellular signal-regulated kinase (ERK) 1/2)], JNK inhibitor II [c-Jun NH 2-terminal kinase (JNK)]. We demonstrated that visfatin could obviously upregulate secretion of MCP-1and IL-6 in a dose- and time-dependent manner in human umbilical vein endothelial cells. Visfatin-induced effects were diminished by SB203580, wortmannin, and PD98059. In summary, these results suggest that visfatin-induced MCP-1 and IL-6 production involve p38 MAPK, PI3K, and ERK 1/2 pathways in human umbilical vein endothelial cells as determined by inhibition with specific inhibitors.

References

Correspondence

Prof. S. B. Qiao, MD 

Department of Cardiology

Cardiovascular Institute and Fuwai Hospital

Chinese Academy of Medical Sciences and Peking Union Medical College

167 BeiLiShi Rd

100037 Beijing

China

Phone: 86/10/8839 88 62

Fax: 86/10/8839 88 62

Email: [email protected]