TumorDiagnostik & Therapie 2009; 30(1): 25-31
DOI: 10.1055/s-0028-1109215
Thieme Onkologie aktuell

© Georg Thieme Verlag KG Stuttgart · New York

Differential Expression of IGF-I and Insulin Receptor Isoforms in HPV Positive and Negative Human Cervical Cancer Cell Lines

M.-L. Serrano1, 2 , M. Sánchez-Gómez2 , M.-M. Bravo1 , S. Yakar3 , D. LeRoith3
  • 1Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá, Colombia
  • 2Laboratorio de Hormonas, Departamento de Química, Universidad Nacional de Colombia, Bogotá, Colombia
  • 3Division of Endocrinology, Department of Medicine, Mount Sinai School of Medicine, New York, USA
Further Information

Publication History

eingereicht: 27.03.2008

angenommen: 23.06.2008

Publication Date:
12 February 2009 (online)


Human papillomavirus (HPV) is the main risk factor for cervical cancer; however, some carcinomas occur in the absence of the virus. IGF-IR and an isoform of the insulin receptor, IR-A, play important roles in cancer. In this study we assessed the role of the IGF/insulin receptors in cervical cancer cell lines with different HPV status, SiHa (HPV positive), and C33a (HPV negative). Different patterns of receptor expression were found; while SiHa expressed IGF-IR, IR-A and IR-B, and IR/IGF-IR hybrid receptors, C33a cells expressed the IR-A only. Tyrosine phosphorylation of these receptors in response to their corresponding ligands correlated with the expression level of these receptors in the cell lines. Activation of PI3-K and MAPK pathways was revealed in both cell lines, however, no effects on proliferation, migration, or invasion were observed. Here we show that cervical cancer cell lines – positive and negative for HPV – differ in the type of insulin and IGF-1 receptors expressed. Additional studies are needed for characterization of the role of IR-A in cervical carcinogenesis.


D. LeRoith,, MD, PhD 

Director of the Metabolism Institute

Chief, Division of Endocrinology, Diabetes & Bone Disease

Department of Medicine

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Email: derek.leroith@mssm.edu