Horm Metab Res 2009; 41(4): 339-341
DOI: 10.1055/s-0028-1112114
Short Communication

© Georg Thieme Verlag KG Stuttgart · New York

Time Course of Effects of Inhalation Anesthesia on Blood Glucose Level in Male and Female C57BL/6 Mice

J. L. Durand 1 , W. Hosinking 1 , L. A. Jelicks 1
  • 1Department of Physiology & Biophysics, Albert Einstein College of Medicine, Bronx, New York, USA
Further Information

Publication History

received 13.08.2008

accepted 17.11.2008

Publication Date:
15 January 2009 (online)


Mice are the most common animal model used for in vivo studies relevant to human disease. The numerous mouse phenotypes available, created by genetic manipulation, enable the linking of specific genes to normal or abnormal molecular and organ functions. Isoflurane is a very common type of anesthesia used in many laboratories performing metabolism and diabetes research. The study of diabetes and obesity focuses on the glucose level as one of the main variables. Moreover, microPET imaging techniques specifically focus on blood glucose biodistribution [1] [2] [3] [4]. The handling and preparation of mice (stress, body temperature, and anesthesia) are very important factors that can impact on glucose levels. Pomplun et al. [5] have previously reported that blood glucose levels in wild type C57Bl/6 mice were significantly elevated immediately following isoflurane anesthesia while Fueger et al. [6] reported that isoflurane anesthesia (2% in 100% oxygen) mildly increased blood glucose levels in severe combined immunodeficient (SCID) male mice [5] [6]. Other investigators have reported development of hypoglycemia during isoflurane anesthesia [1] [2] [7].

Since we routinely use isoflurane, a common type of inhalation anesthesia, in our imaging studies we examined its impact on blood glucose levels in male and female mice. Mice exhibited an elevation in blood glucose levels immediately after the period of anesthesia. This effect was significant for male mice. Subsequently, glucose readings declined significantly, and eventually attained a reading very similar to the initial reading, pre-anesthesia.


  • 1 Dandekar M, Tseng JR, Gambhir SS. J Nucl Med. 2007;  48 602-607
  • 2 Flores JE, MacFarland LM, Vanderbilt A, Ogasawara AK, Williams SP. Mol Imaging Biol. 2008;  10 192-200
  • 3 Toyama H, Ichise M, Liow JS, Modell KJ, Vines DC, Esaki T, Cook M, Seidel J, Sokoloff L, Green MV, Innis RB. J Nucl Med. 2004;  45 1398-1405
  • 4 Woo SK, Lee TS, Kim KM, Kim JY, Jung JH, Kang JH, Cheon GJ, Choi CW, Lim SM. Nucl Med Biol. 2008;  35 143-150
  • 5 Pomplun D, Mohlig M, Spranger J, Pfeiffer AF, Ristow M. Horm Metab Res. 2004;  36 67-69
  • 6 Fueger BJ, Czernin J, Hildebrandt I, Tran C, Halpern BS, Stout D, Phelps ME, Weber WA. J Nucl Med. 2006;  47 999-1006
  • 7 Loepke AW, MacCann JC, Kurth CD, McAuliffe JJ. Anesth Analg. 2006;  102 75-80
  • 8 Illera JC, Gonzalez Gil A, Silvan G, Illera M. J Physiol Biochem. 2000;  56 329-336


J. L. Durand

Department of Physiology & Biophysics

Albert Einstein College of Medicine


10461 New York


Phone: 778/430 32 57/819

Fax: 778/430 32 57/819

Email: [email protected]