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Exp Clin Endocrinol Diabetes 1983; 82(5): 188-193
DOI: 10.1055/s-0029-1210275
Original

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

A Simple Solid Phase Enzyme Immunoassay for Aldosterone in Plasma and Saliva1)

W. Hubl, H. Taubebt, E. Freymann, F. Hofmann, D. Meissner, C. D. Garten, P. K. H. Schmidt, H. J. Thiele, B. Neef
  • Institute of Clinical Chemistry and Laboratory Diagnosis (Head: Dr. rer. nat. D. Meißner),
    Department of Heart Diseases (Head: MR Doz. Dr. sc. med. P. K. H. Schmidt) and IIIrd Department of Medicine of Dresden-Friedrichstadt Hospital (Director: OMR Prof. Dr. sc. med. O. Günther),
    Institute for Medical Diagnosis (Director: MR Dr. Dr. sc. med. H. J. Thiele) and VEB Sächsisches Serumwerk Dresden (Director: Obering. M. Kluge) Dresden/GDR
1) Supported by the Research Project of Medizinische Laboratoriumsdiagnostik of the Ministry of Health/GDR.
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Publication History

1982

Publication Date:
17 July 2009 (online)

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Summary

A sensitive, solid phase enzyme immunoassay for the determination of aldosterone in plasma and saliva has been developed.

For this purpose anti-aldosterone coated tubes were used. The enzyme marker was a covalently linked aldosterone/horseradish peroxidase conjugate. The assay had a limit of detection of 5 pg/ assay tube. The test requires neither centrifuge nor gamma or beta counter, but only a simple photometer. The free/bound separation is based on the solid phase technique with a simple washing step.

The normal values for aldosterone in plasma and saliva before and after ACTH application were determined. Saliva, being easily collected by a non-invasive, stress-free technique, is an alternative to plasma. The aldosterone levels in patients with Conn's syndrome were significantly increased in plasma and also in saliva.

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