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Exp Clin Endocrinol Diabetes 1988; 91(1): 13-19
DOI: 10.1055/s-0029-1210716
Original

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Screening Monoclonal Islet Cell Surface Antibodies (ICSA) by Radioimmunoassay — Detection of Crossreactivity with ICSA from Insulin-Dependent (Type 1) Diabetic Patients1)

H. Keilacker, B. Ziegler, J. M. Diaz-Alonso, S. Witt, K.-P. Woltanski, K.-D. Kohnert, M. Ziegler
  • Central Institute of Diabetes “Gerhardt Katsch” (Director: OMR Prof. Dr. sc. med. H. Bibergeil), Karlsburg/GDR
1) Dedicated to Professor Gerhardt Katsch (1887—1961) on the occasion of his 100th birthday.
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1987

Publikationsdatum:
16. Juli 2009 (online)

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Summary

A radioimmunoassay for the detection of monoclonal islet cell antibodies was developed using rat insulinoma cells as antigen carriers and 125I-labeled affinity-chromatographically purified anti-mouse Ig antibodies for detecting cell-bound mouse 1g. Prior to the assay cells had been attached to glass tubes by poly-dimethyl-diallyl ammonium chloride thus allowing to perform the assay as easy as a solid-phase immunoassay. Incubation protocol and cell number were chosen to ensure a high sensitivity of the assay. Results compared well with immunofluorescence findings. 0f seven monoclonal islet cell antibodies tested for crossreactivity only one was displaceable by islet cell surface antibodies from diabetic sera. This antibody was induced by immunization with human islets whereas all others were from mice which had been autoimmunized with streptozotocin and complete Freund's adjuvant.

Key words

Monoclonal islet cell surface antibodies - Radioimmunoassay - RIN cells - Displacement by human ICSA