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Exp Clin Endocrinol Diabetes 1989; 94(4/05): 151-156
DOI: 10.1055/s-0029-1210891
Original

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Arginine-Vasopressin Binding to Isolated Hippocampal Microvessels of Rats with Different Endogenous Concentrations of the Neuropeptide*)

Roswitha Kretzschmar, A. Ermisch
  • Dept. of Cell Biology and Regulation (Head: Prof. Dr. sc. nat. A. Ermisch), Section of Biosciences (Head: Prof. Dr. sc. nat. A. Ermisch), Karl-Marx-University, Leipzig, GDR
*) Dedicated to Professor Dr. G. Dörner on the occasion of his 60th birthday.
Further Information

Publication History

1988

Publication Date:
16 July 2009 (online)

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Summary

The binding of [125I]arginine.8-vasopressin (AVP) to hippocampal microvessels isolated from brains of normal Wistar rats, animals after water deprivation and heterozygous as well as homozygous diabetes-insipidus rats (Brattleboro strain) were measured. Data from binding experiments from the microvessels of the different groups of animals in each case revealed a single class of high affinity binding sites. However, the binding parameters between the different groups of rats were different. The affinity constants differs by a factor of 5.1, ranging from KD = 1.18 nmol × L−1 (animals after water deprivation) to KD = 6.05 nmol × L−1 (homozygous Brattleboro rats). The binding capacity, differing by a factor of 3.5, ranged from Bmax = 245 fmol × mg−1 to Bmax = 865 fmol × mg−1.

Key words

Arginine-vasopressin - Radioligand-binding-studies - AVP binding sites - Isolated microvessels - Blood-brain barrier - Regulation of AVP binding

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