Horm Metab Res 2009; 41(12): 866-873
DOI: 10.1055/s-0029-1233457
Animals, Clinical

© Georg Thieme Verlag KG Stuttgart · New York

Temporal Evaluation of Body Composition, Glucose Homeostasis and Lipid Profile of Male Rats Programmed by Maternal Protein Restriction During Lactation

A. T. S. Fagundes1 , E. G. Moura1 , M. C. F. Passos2 , A. P. Santos-Silva1 , E. de Oliveira1 , I. H. Trevenzoli1 , G. Casimiro-Lopes1 , J. F. Nogueira-Neto3 , P. C. Lisboa1
  • 1Departamento de Ciências Fisiológicas, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil
  • 2Departamento de Nutrição Aplicada, Instituto de Nutrição, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil
  • 3Laboratório de Lipídeos, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil
Further Information

Publication History

received 13.01.2009

accepted 15.06.2009

Publication Date:
11 August 2009 (online)

Abstract

Neonatal protein restriction causes lower body weight and hormonal dysfunctions in 6 months-old rats. In this model, we studied the body composition, glycogen content, serum lipid, serum protein, and hormones related to glucose homeostasis in the offspring during development. At birth, lactating rats were divided into: control dams – fed a normal diet (23% protein) and protein restricted dams – fed a diet with 8% protein. After weaning, pups received normal diet. Offspring were killed at 21, 90, and 180 days-old. Protein restricted offspring showed lower visceral fat (90th day: 14%; 180th day: 19%) and lower total fat (90th day: 16%; 180th day: 14%) that explain their lower body weight. They presented lower glycemia (180th day: 17%), lower insulinemia (21st day: 63%; 180th day: 24%), higher adiponectinemia (21st day: 169%), higher liver glycogen (21st day: 104%), and higher muscle glycogen (180th day: 106%), suggesting a higher insulin sensitivity. The higher serum corticosterone (50%), higher adrenal total catecholamines content (98%) as well as in vitro catecholamine secretion (26%) of adult protein restricted offspring, suggest a programming stimulatory effect upon adrenal gland. They also presented several biochemical changes, such as lower serum total protein, albumin and globulin (21st day: 17, 21, 12%, respectively), higher LDL-c (21st day: 69%), lower triglycerides (21st day: 42%; 90th day: 39%), and lower total cholesterol (180th day: 16%). Thus, maternal protein restriction during lactation induces an energy-protein malnutrition, characterized by an impairment of the pup's protein anabolism and, after weaning, the lower adiposity suggests lower lipogenesis and higher lipolytic activity, probably caused by catecholamine and glucocorticoid action.

References

Correspondence

Dr. P. C. Lisboa

Departamento de Ciências Fisiológicas – 5° andar

Instituto de Biologia

Universidade do Estado do Rio de Janeiro

Av. 28 de setembro

87- Rio de Janeiro

RJ 20551-030

Brazil

Phone: +21/2587/61 34

Fax: 21/2587/61 29

Email: [email protected]

Email: [email protected]