Legionella pneumophila induced microRNA expression in human lung epithelial cells

B Schmeck; B Dolniak; I Pollock; C Schulz; W Bertrams; A Flieger; N Suttorp; S Hippenstiel

doi:10.1055/s-0030-1251242

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Pneumologie 2010; 64 - P400
DOI: 10.1055/s-0030-1251242

Legionella pneumophila induced microRNA expression in human lung epithelial cells

B Schmeck ¹, B Dolniak ¹, I Pollock ¹, C Schulz ¹, W Bertrams ¹, A Flieger ², N Suttorp ³, S Hippenstiel ³

¹Charité-Universitätsmedizin Berlin, Med. Klinik m.S. Infektiologie und Pneumologie BMBF-Research Group „Systems Biology of Lung Inflammation (FORSYS-Lung)“
²Robert Koch-Institut, FG11 Abteilung für Bakterielle Infektionen
³Charité-Universitätsmedizin Berlin Med. Klinik m.S. Infektiologie und Pneumologie

Congress Abstract

Legionella pneumophila cause severe community- and hospital-acquired pneumonia. Lung airway and alveolar epithelial cells comprise an important sentinel system in airborne infections. However, little is known on specific Legionella-induced signal transduction in the lung, especially the effect on chromatin remodeling and miRNA expression are widely unknown. In this study we performed a comprehensive analysis of miRNAs in L. pneumophila-infected lung epithelial cells by Next Generation Sequencing (Solexa Illumnia) and characterized chromatin remodeling on distinct gene promoters by quantitative Chromatin Immunoprecipitation (ChIP). Data are currently analyzed and prepared for systems biology modeling of the host-pathogen interplay.