Int J Sports Med 2010; 31(9): 599-603
DOI: 10.1055/s-0030-1255107
Physiology & Biochemistry

© Georg Thieme Verlag KG Stuttgart · New York

Blood and Salivary Oxidative Stress Biomarkers Following an Acute Session of Resistance Exercise in Humans

R. Deminice1 , T. Sicchieri2 , P. O. Payão1 , A. A. Jordão1
  • 1Nutrition and Metabolism, University of Sao Paulo, Ribeirão Preto, Brazil
  • 2Exercise Physiology, Federal University of São Carlos, Brazil
Further Information

Publication History

accepted after revision May 20, 2010

Publication Date:
08 July 2010 (online)

Abstract

The aim of the present study was to compare oxidative stress biomarkers determined in blood and saliva before and after acute resistance exercise. 1 week after 1 maximum repetition (1RM) test 11 healthy well-trained males completed a hypertrophy acute session of resistance training including 3 sets of 10 repetitions at 75% of the 1RM, with 90 s rest periods between sets. Venous blood and saliva samples were collected before (pre) and 10 min after (post) the resistance training session. A significant (p<0.05) rise in blood lactate accumulation (pre: 1.6±0.4 vs. post: 9.5±2.4) was found post-acute resistance training compared with baseline values. Significant increases (p<0.05) in TBARS (42%), AOPP (28%), uric acid (27%) and GSH (14%) were detected post-acute resistance training in relation to pre in blood samples. A significant increase (p<0.05) in uric acid (36%) was found in saliva post-acute resistance training as well as a significant correlation (p<0.05) between uric acid determined in blood and saliva. Statistical analysis did not reveal any other change in the salivary oxidative stress biomarkers. In conclusion, an acute session of resistance exercise induces oxidative stress in plasma of trained men after acute resistance training, which was not found in saliva samples except for uric acid.

References

  • 1 Aizenbud D, Peri-Front Y, Nagler RM. Salivary analysis and antioxidants in cleft lip and palate children.  Arch Oral Biol. 2008;  53 517-522
  • 2 American College of Sports Medicine . American College of Sports Medicine position stand. Progression models in resistance training for healthy adults.  Med Sci Sports Exerc. 2009;  41 687-708
  • 3 Arana C, Cutando A, Ferrera MJ, Gómez-Moreno G, Worf CV, Bolaños MJ, Escames G, Acuña-Castroviejo D. Parameters of oxidative stress in saliva from diabetic and parenteral drug addict patients.  J Oral Pathol Med. 2006;  35 554-559
  • 4 Bahar G, Feinmesser R, Shpitzer T, Popovtzer A, Nagler RM. Salivary analysis in oral cancer patients: DNA and protein oxidation, reactive nitrogen species, and antioxidant profile.  Cancer. 2007;  109 54-59
  • 5 Battino M, Ferreiro MS, Gallardo I, Newman HN, Bullon P. The antioxidant capacity of saliva.  J Clin Periodontol. 2002;  29 189-194
  • 6 Beaven CM, Gill ND, Cook CJ. Salivary testosterone and cortisol responses in professional rugby players after 4 resistance exercise protocols.  J Strength Cond Res. 2008;  22 426-432
  • 7 Benitez-Sillero JD, Perez-Navero JL, Tasset I, Guillen-Del Castillo M, Gil-Campos M, Tunez I. Influence of intense exercise on saliva glutathione in prepubescent and pubescent boys.  Eur J Appl Physiol. 2009;  106 181-186
  • 8 Bibi G, Green Y, Nagler R. Compositional and oxidative analysis in the saliva and serum of predialysis chronic kidney disease patients and end-stage renal failure patients on peritoneal dialysis.  Ther Apher Dial. 2008;  12 164-170
  • 9 Bloomer RJ. Effect of exercise on oxidative stress biomarkers.  Adv Clin Chem. 2008;  46 1-50
  • 10 Bloomer RJ, Fry AC, Falvo MJ, Moore CA. Protein carbonyls are acutely elevated following single set anaerobic exercise in resistance trained men.  J Sci Med Sport. 2007;  10 411-417
  • 11 Bloomer RJ, Falvo MJ, Fry AC, Schilling BK, Smith WA, Moore CA. Oxidative stress response in trained men following repeated squats or sprints.  Med Sci Sports Exerc. 2006;  38 1436-1442
  • 12 Carrillo AE, Murphy RJ, Cheung SS. Vitamin C supplementation and salivary immune function following exercise-heat stress.  Int J Sports Physiol Perform. 2008;  3 516-530
  • 13 Cavas L, Arpinar P, Yurdakoc K. Possible interactions between antioxidant enzymes and free sialic acids in saliva: a preliminary study on elite judoists.  Int J Sports Med. 2005;  26 832-835
  • 14 Chicharro JL, Lucía A, Pérez M, Vaquero AF, Ureña R. Saliva composition and exercise.  Sports Med. 1998;  26 17-27
  • 15 Costa CM, Dos Santos RCC, Lima E. A simple automated procedure for thiol measurement in human and serum samples.  Braz J Pathol Lab Med. 2006;  42 345-350
  • 16 Crewther B, Cronin J, Keogh J, Cook C. The salivary testosterone and cortisol response to 3 loading schemes.  J Strength Cond Res. 2008;  22 250-255
  • 17 Dietary Reference Intakes: Recommended intakes for individuals .Comprehensive DRI table for methionine, folate, vitamin B12, iron and calcium. National Academy of Sciences, Institute of Medicine, Food and Nutrition Board; 2005
  • 18 Elosua R, Molina L, Fito M, Arquer A, Sanchez-Quesada JL, Covas MI, Ordoñez-Llanos J, Marrugat J. Response of oxidative stress biomarkers to a 16-week aerobic physical activity program, and to acute physical activity, in healthy young men and women.  Atherosclerosis. 2003;  167 327-334
  • 19 González D, Marquina R, Rondón N, Rodriguez-Malaver AJ, Reyes R. Effects of aerobic exercise on uric acid, total antioxidant activity, oxidative stress, and nitric oxide in human saliva.  Res Sports Med. 2008;  16 128-137
  • 20 Guentsch A, Preshaw PM, Bremer-Streck S, Klinger G, Glockmann E, Sigusch BW. Lipid peroxidation and antioxidant activity in saliva of periodontitis patients: effect of smoking and periodontal treatment.  Clin Oral Investig. 2008;  12 345-352
  • 21 Haeckel R, Hänecke P. Application of saliva for drug monitoring. An in vivo model for transmembrane transport.  Eur J Clin Chem Clin Biochem. 1996;  34 171-191
  • 22 Harriss DJ, Atkinson G. International Journal of Sports Medicine – Ethical Standards in Sport and Exercise Science Research.  Int J Sports Med. 2009;  30 701-702
  • 23 Hudson MB, Hosick PA, McCaulley GO, Schrieber L, Wrieden J, McAnulty SR, Triplett NT, McBride JM, Quindry JC. The effect of resistance exercise on humoral markers of oxidative stress.  Med Sci Sports Exerc. 2008;  40 542-548
  • 24 Kondakova I, Lissi EA, Pizarro M. Total reactive antioxidant potential in human saliva of smokers and non-smokers.  Biochem Mol Biol Int. 1999;  47 911-920
  • 25 Kuzkaya N, Weissmann N, Harrison DG, Dikalov S. Interactions of peroxynitrite with uric acid in the presence of ascorbate and thiols: implications for uncoupling endothelial nitric oxide synthase.  Biochem Pharmacol. 2005;  70 343-354
  • 26 Lima AO, Soares JB, Greco JB, Galizzi J, Cancardo JR. Methods Applied to the Clinical Laboratory, Rio de Janeiro, Guanabara-Koogan.  1992; 
  • 27 Michailidis Y, Jamurtas AZ, Nikolaidis MG, Fatouros IG, Koutedakis Y, Papassotiriou I, Kouretas D. Sampling time is crucial for measurement of aerobic exercise-induced oxidative stress.  Med Sci Sports Exerc. 2007;  39 1107-1113
  • 28 Nagler RM. Altered salivary profile in heavy smokers and its possible connection to oral cancer.  Int J Biol Markers. 2007;  22 274-280
  • 29 Nikolaidis MG, Jamurtas AZ, Paschalis V, Fatouros IG, Koutedakis Y, Kouretas D. The effect of muscle-damaging exercise on blood and skeletal muscle oxidative stress: magnitude and time-course considerations.  Sports Med. 2008;  38 579-606
  • 30 Pacque PF, Booth CK, Ball MJ, Dwyer DB. The effect of an ultra-endurance running race on mucosal and humoral immune function.  J Sports Med Phys Fitness. 2007;  47 496-501
  • 31 Pérez M, Lucía A, Carvajal A, Pardo J, Chicharro JL. Determination of the maximum steady state of lactate (MLSS) in saliva: an alternative to blood lactate determination.  Jpn J Physiol. 1999;  49 395-400
  • 32 Södergren E, Nourooz-Zadeh J, Berglund L, Vessby B. Re-evaluation of the ferrous oxidation in xylenol orange assay for the measurement of plasma lipid hydroperoxides.  J Biochem Biophys Methods. 1998;  37 137-146
  • 33 Witko-Sarsat V, Friedlander M, Capeillère-Blandin C, Nguyen-Khoa T, Nguyen AT, Zingraff J, Jungers P, Descamps-Latscha B. Advanced oxidation protein products as a novel marker of oxidative stress in uremia.  Kidney Int. 1996;  49 1304-1313
  • 34 Wong DT. Salivary diagnostics powered by nanotechnologies, proteomics and genomics.  J Am Dent Assoc. 2006;  137 313-321
  • 35 Zloczower M, Reznick AZ, Zouby RO, Nagler RM. Relationship of flow rate, uric acid, peroxidase, and superoxide dismutase activity levels with complications in diabetic patients: can saliva be used to diagnose diabetes?.  Antioxid Redox Signal. 2007;  9 765-773

Correspondence

Rafael Deminice

Nutrition and Metabolism

University of Sao Paulo

Ribeirão Preto

Brazil

Phone: +05/516/3602 4564

Fax: +05/516/3602 4564

Email: deminice@ig.com.br

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