Horm Metab Res 2010; 42(10): 754-757
DOI: 10.1055/s-0030-1262837
Short Communication

© Georg Thieme Verlag KG Stuttgart · New York

Vitamin D Regulates Steroidogenesis and Insulin-like Growth Factor Binding Protein-1 (IGFBP-1) Production in Human Ovarian Cells

G. Parikh1 , M. Varadinova1 , P. Suwandhi1 , T. Araki1 , Z. Rosenwaks2 , L. Poretsky1 , D. Seto-Young1
  • 1G. J. Friedman Diabetes Institute and Division of Endocrinology, Department of Medicine, Beth Israel Medical Center, Albert Einstein College of Medicine, New York, USA
  • 2Center for Reproductive Medicine and Infertility, Weill Medical College of Cornell University, New York, USA
Weitere Informationen


received 19.04.2010

accepted 12.07.2010

13. August 2010 (online)


Vitamin D Receptor (VDR) is expressed in both animal and human ovarian tissue, however, the role of vitamin D in human ovarian steroidogenesis is unknown. Cultured human ovarian cells were incubated in tissue culture medium supplemented with appropriate substrates, with or without 50 pM–150 pM or 50 nM−150 nM of 1,25-(OH)2D3, and in the presence or absence of insulin. Progesterone, testosterone, estrone, estradiol, and IGFBP-1 concentrations in conditioned tissue culture medium were measured. Vitamin D receptor was present in human ovarian cells. 1,25-(OH)2D3 stimulated progesterone production by 13% (p<0.001), estradiol production by 9% (p<0.02), and estrone production by 21% (p<0.002). Insulin and 1,25-(OH)2D3 acted synergistically to increase estradiol production by 60% (p<0.005). 1,25-(OH)2D3 alone stimulated IGFBP-1 production by 24% (p<0.001), however, in the presence of insulin, 1,25-(OH)2D3 enhanced insulin-induced inhibition of IGFBP-1 production by 13% (p<0.009). Vitamin D stimulates ovarian steroidogenesis and IGFBP-1 production in human ovarian cells likely acting via vitamin D receptor. Insulin and vitamin D synergistically stimulate estradiol production. Vitamin D also enhances inhibitory effect of insulin on IGFBP-1 production.


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D. Seto-YoungPhD 

Division of Endocrinology

Beth Israel Medical Center

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