Horm Metab Res 2011; 43(1): 37-42
DOI: 10.1055/s-0030-1268458
Animals

© Georg Thieme Verlag KG Stuttgart · New York

Study on the Effect of Peripheral Kisspeptin Administration on Basal and Glucose-induced Insulin Secretion Under Fed and Fasting Conditions in the Adult Male Rhesus Monkey (Macaca mulatta)

F. Wahab1 , T. Riaz1 , M. Shahab1
  • 1Laboratory of Reproductive Neuroendocrinology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Further Information

Publication History

received 07.06.2010

accepted 26.10.2010

Publication Date:
06 December 2010 (eFirst)

Abstract

Kisspeptin (KP)-Kiss1r, a ligand-receptor pair, has recently been implicated as a pivotal regulator of the neuroendocrine reproductive axis. Kiss1 (encoding KP) as well as Kiss1r (encoding receptor for KP) are expressed in several peripheral tissues including the pancreas. But the specific role of KP in the physiology of pancreas is still incompletely deciphered. This study was designed to examine the effect of peripheral KP administration on basal and glucose-induced plasma insulin (an important pancreatic hormone) secretion under fed and fasting conditions in the adult male rhesus monkey. A set of 4 chair-restraint habituated intact adult male rhesus monkeys were assigned to receive intravenous bolus administration of human kisspeptin-10 (KP10, 50 μg), and vehicle (1 ml) in normal fed and fasting conditions without or with glucose infusions. Plasma concentrations of insulin were measured by using a specific IRMA. Glucose infusion significantly stimulated plasma insulin levels (p<0.005). Vehicle administration did not affect both basal and glucose stimulated insulin in fed as well as in fasting condition. KP10 administration had no effect on the basal insulin levels in both fed and fasting as compared to pretreatment or vehicle treatment levels, while it significantly heightened glucose stimulated insulin levels (p<0.05) in both fed and fasted monkeys. The present results show that KP administration does not affect the basal secretion of insulin under both fed and fasting condition while potentiated the glucose-induced insulin levels in the adult male rhesus monkey. Therefore, these findings suggest a potential role of KP in the physiology of pancreas.

References

Correspondence

Dr. M. Shahab

Reproductive Neuroendocrinology

Laboratory

Department of Animal Sciences

Faculty of Biological Sciences

Quaid-i-Azam University

Islamabad

Pakistan

Phone: +92/51/90643014

Fax: +92/51/2601176

Email: Shahab@qau.edu.pk