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Int J Sports Med 2011; 32(4): 277-280
DOI: 10.1055/s-0030-1269922
Training & Testing

© Georg Thieme Verlag KG Stuttgart · New York

Reproducibility of Muscle Oxygen Saturation

C. Thiel1 , L. Vogt1 , H. Himmelreich2 , M. Hübscher1 , W. Banzer1
  • 1Goethe University, Sports Medicine, Frankfurt, Germany
  • 2Goethe University Hospital, Trauma, Hand and Reconstructive Surgery, Frankfurt, Germany
Further Information

Publication History

accepted after revision November 24, 2010

Publication Date:
26 January 2011 (online)

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Abstract

The present study evaluated the reproducibility of tissue oxygenation in relation to oxygen consumption (VO2) across cycle exercise intensities in a test-retest design. 12 subjects (25.7±2.1 years; 24.7±1.9 kg · m−2) twice performed an incremental bicycle exercise protocol, while tissue oxygen saturation (StO2) in the vastus lateralis muscle was monitored by a commercially available NIRS unit and VO2 determined by an open-circuit indirect calorimetric system. Coefficients of variation across rest, workloads corresponding to 25, 50 and 75% of individual maximum capacity, and maximum load were 5.8, 4.6, 6.1, 8.0, 11.0% (StO2) and 7.6, 6.0, 3.7, 3.4, 3.1% (VO2), respectively. 95 % CI of relative test-retest differences ranged from −5.6 to +5.4% at 25% load to −17.2 to +7.5% at maximum load for StO2 and from −7.3 to +7.7% at rest to −3.3 to +3.2% at maximum load for VO2. With advancing exercise intensity, within-subject variability of StO2 was augmented, whereas VO2 variability slightly attenuated. NIRS measurements at higher workloads need to be interpreted with caution.

Key words

measurement error - reliability - near-infrared spectroscopy (NIRS) - muscle oxygen saturation - oxygen consumption

References

  • 1 Armstrong LE, Costill DL. Variability of respiration and metabolism: responses to submaximal cycling and running.  Res Q Exerc Sport. 1985;  56 93-96
    PubMedGoogle Scholar
  • 2 Atkinson G, Nevill AM. Statistical methods for assessing measurement error (reliability) in variables relevant to sports medicine.  Sports Med. 1998;  26 217-238
    CrossrefPubMedGoogle Scholar
  • 3 Austin KG, Daigle KA, Patterson P, Cowman J, Chelland S, Haymes EM. Reliability of near-infrared spectroscopy for determining muscle oxygen saturation during exercise.  Res Q Exerc Sport. 2005;  76 440-449
    CrossrefPubMedGoogle Scholar
  • 4 Bassett DR, Howley ET. Limiting factors for maximum oxygen uptake and determinants of endurance performance.  Med Sci Sports Exerc. 2000;  32 70-84
    PubMedGoogle Scholar
  • 5 Becque MD, Katch V, Marks C, Dyer R. Reliability and within subject variability of VE, VO2, heart rate and blood pressure during submaximum cycle ergometry.  Int J Sports Med. 1993;  14 220-223
    Article in Thieme ConnectPubMedGoogle Scholar
  • 6 Belardinelli R, Barstow TJ, Porszasz J, Wasserman K. Changes in skeletal muscle oxygenation during incremental exercise measured with near infrared spectroscopy.  Eur J Appl Physiol. 1995;  70 487-492
    CrossrefPubMedGoogle Scholar
  • 7 Boushel R, Langberg H, Olesen J, Gonzales-Alonzo J, Bülow J, Kjaer M. Monitoring tissue oxygen availability with near infrared spectroscopy (NIRS) in health and disease.  Scand J Med Sci Sports. 2001;  11 213-222
    CrossrefPubMedGoogle Scholar
  • 8 Buono MJ, Miller PW, Hom C, Pozos RS, Kolkhorst FW. Skin blood flow affects in vivo near-infrared spectroscopy measurements in human skeletal muscle.  Jpn J Physiol. 2005;  55 241-244
    CrossrefPubMedGoogle Scholar
  • 9 Carter J, Jeukendrup AE. Validity and reliability of three commercially available breath-by-breath respiratory systems.  Eur J Appl Physiol. 2002;  86 435-441
    CrossrefPubMedGoogle Scholar
  • 10 Creteur J. Muscle StO2 in critically ill patients.  Curr Opin Crit Care. 2008;  14 361-366
    CrossrefPubMedGoogle Scholar
  • 11 Davies RC, Eston RG, Poole DC, Rowlands AV, DiMenna F, Wilkerson DP, Twist C, Jones AM. Effect of eccentric exercise-induced muscle damage on the dynamics of muscle oxygenation and pulmonary oxygen uptake.  J Appl Physiol. 2008;  105 1413-1421
    CrossrefPubMedGoogle Scholar
  • 12 Davis SL, Fadel PJ, Cui J, Thomas GD, Crandall CG. Skin blood flow influences near-infrared spectroscopy-derived measurements of tissue oxygenation during heat stress.  J Appl Physiol. 2006;  100 221-224
    CrossrefPubMedGoogle Scholar
  • 13 Ferrari M, Mottola L, Quaresima V. Principles, techniques, and limitations of near infrared spectroscopy.  Can J Appl Physiol. 2004;  29 463-487
    CrossrefPubMedGoogle Scholar
  • 14 Gallagher D, Heymsfield SB, Heo M, Jebb SA, Murgatroyd PR, Sakamoto Y. Healthy percentage body fat ranges: an approach for developing guidelines based on body mass index.  Scand J Med Sci Sports. 2000;  72 694-701
    PubMedGoogle Scholar
  • 15 Harriss DJ, Atkinson G. International Journal of Sports Medicine – Ethical Standards in Sport and Exercise Science Research.  Int J Sports Med. 2009;  30 701-702
    Google Scholar
  • 16 Hodges LD, Brodie DA, Bromley PD. Validity and reliability of selected commercially available metabolic analyzer systems.  Scand J Med Sci Sports. 2005;  15 271-279
    CrossrefPubMedGoogle Scholar
  • 17 Ikossi DG, Knudson MM, Morabito DJ, Cohen MJ, Wan JJ, Khaw L, Stewart CJ, Hemphill C, Manley GT. Continuous muscle tissue oxygenation in critically injured patients: a prospective observational study.  J Trauma. 2006;  61 780-788 discussion 788–790
    CrossrefPubMedGoogle Scholar
  • 18 Kalliokoski KK, Scheede-Bergdahl C, Kjaer M, Boushel R. Muscle perfusion and metabolic heterogeneity: insights from noninvasive imaging techniques.  Exerc Sport Sci Rev. 2006;  34 164-170
    CrossrefPubMedGoogle Scholar
  • 19 Katayama K, Yoshitake Y, Watanabe K, Akima H, Ishida K. Muscle deoxygenation during sustained and intermittent isometric exercise in hypoxia.  Med Sci Sports Exerc. 2009;  42 1269-1278
    PubMedGoogle Scholar
  • 20 Kennedy MD, Haykowsky MJ, Boliek CA, Esch BTA, Scott JM, Warburton DER. Regional muscle oxygenation differences in vastus lateralis during different modes of incremental exercise.  Dyn Med. 2006;  5 8
    CrossrefPubMedGoogle Scholar
  • 21 Koga S, Poole DC, Ferreira LF, Whipp BJ, Kondo N, Saitoh T, Ohmae E, Barstow TJ. Spatial heterogeneity of quadriceps muscle deoxygenation kinetics during cycle exercise.  J Appl Physiol. 2007;  103 2049-2056
    CrossrefPubMedGoogle Scholar
  • 22 Lucía A, Fleck SJ, Gotshall RW, Kearney JT. Validity and reliability of the Cosmed K2 instrument.  Int J Sports Med. 1993;  14 380-386
    Article in Thieme ConnectPubMedGoogle Scholar
  • 23 Macfarlane DJ. Automated metabolic gas analysis systems: a review.  Sports Med. 2001;  31 841-861
    CrossrefPubMedGoogle Scholar
  • 24 Mancini DM, Bolinger L, Li H, Kendrick K, Chance B, Wilson JR. Validation of near-infrared spectroscopy in humans.  J Appl Physiol. 1994;  77 2740-2747
    PubMedGoogle Scholar
  • 25 Meglinski IV, Matcher SJ. Computer simulation of the skin reflectance spectra.  Comput Methods Programs Biomed. 2003;  70 179-186
    CrossrefPubMedGoogle Scholar
  • 26 Poole DC, Barstow TJ, McDonough P, Jones AM. Control of oxygen uptake during exercise.  Med Sci Sports Exerc. 2008;  40 462-474
    CrossrefPubMedGoogle Scholar
  • 27 Snyder AC, Parmenter MA. Using near-infrared spectroscopy to determine maximal steady state exercise intensity.  J Strength Cond Res. 2009;  23 1833-1840
    CrossrefPubMedGoogle Scholar
  • 28 Sucec A, Wicker E. Moderate hypoxia (1 880 m) increases arterial oxygen saturation variability from rest to maximal exercise.  Med Sci Sports Exerc. 2005;  37 S296
    PubMedGoogle Scholar
  • 29 Unnithan VB, Murray LA, Timmons JA, Buchanan D, Paton JY. Reproducibility of cardiorespiratory measurements during submaximal and maximal running in children.  Br J Sports Med. 1995;  29 66-71
    CrossrefPubMedGoogle Scholar
  • 30 van Beekvelt MC, Borghuis MS, van Engelen BG, Wevers RA, Colier WN. Adipose tissue thickness affects in vivo quantitative near-IR spectroscopy in human skeletal muscle.  Scand J Med Sci Sports. 2001;  101 21-28
    PubMedGoogle Scholar
  • 31 van Beekvelt MC, Colier WN, Wevers RA, van Engelen BG. Performance of near-infrared spectroscopy in measuring local O(2) consumption and blood flow in skeletal muscle.  J Appl Physiol. 2001;  90 511-519
    PubMedGoogle Scholar
  • 32 van den Brand JGH, Verleisdonk EJMM, van der Werken C. Near infrared spectroscopy in the diagnosis of chronic exertional compartment syndrome.  Am J Sports Med. 2004;  32 452-456
    CrossrefPubMedGoogle Scholar
  • 33 Vilhena Mendonça G de, Pereira FD. Between-day variability of net and gross oxygen uptake during graded treadmill walking: effects of different walking intensities on the reliability of locomotion economy.  Appl Physiol Nutr Metabol. 2008;  33 1199-1206
    PubMedGoogle Scholar
  • 34 Wassenaar EB, van den Brand JGH. Reliability of near-infrared spectroscopy in people with dark skin pigmentation.  J Clin Monit Comput. 2005;  19 195-199
    CrossrefPubMedGoogle Scholar
  • 35 Wergel-Kolmert U, Wisén A, Wohlfart B. Repeatability of measurements of oxygen consumption, heart rate and Borg's scale in men during ergometer cycling.  Clin Physiol Funct Imaging. 2002;  22 261-265
    CrossrefPubMedGoogle Scholar
  • 36 Wilmore JH, Stanforth PR, Turley KR, Gagnon J, Daw EW, Leon AS, Rao DC, Skinner JS, Bouchard C. Reproducibility of cardiovascular, respiratory, and metabolic responses to submaximal exercise: the HERITAGE Family Study.  Med Sci Sports Exerc. 1998;  30 259-265
    PubMedGoogle Scholar
  • 37 Withers RT, Gore CJ, Gass G, Hahn A. Determination of maximal oxygen consumption (VO2 max) or maximal aerobic power. In: Gore CJ, (ed) Physiological Tests for Elite Athletes. Champaign: Human Kinetics; 2000: 114-127
    Google Scholar

Correspondence

Dr. Christian Thiel

Goethe University

Sports Medicine

Ginnheimer Landstraße 39

60487 Frankfurt

Germany

Phone: +49/69/798 24506

Fax: +49/69/798 24592

Email: c.thiel@sport.uni-frankfurt.de

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