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Horm Metab Res 2011; 43(2): 92-99
DOI: 10.1055/s-0030-1270451
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Presence of Hyperemic Islets in Human Donor-Pancreata Results in Reduced Islet Isolation Yield

D. E. Hilling1 , H. A. M. Töns1 , P. J. Marang-van de Mheen2 , A. G. Baranski1 , E. K. van den Akker1 , O. T. Terpstra1 , E. Bouwman1
  • 1Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands
  • 2Department of Medical Decision Making, Leiden University Medical Center, Leiden, The Netherlands
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Publication History

received 30.08.2010

accepted 02.12.2010

Publication Date:
13 January 2011 (online)

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Abstract

When studying histological characteristics of human donor-pancreata, a remarkably high number of hyperemic islets (HIs) were encountered. The abnormalities in these HIs ranged from single/multiple dilated vessels to hemorrhages extending into the exocrine tissue. We aimed to determine the relevance of the presence of HIs in human donor-pancreata for isolation outcome and to identify donor and procurement factors associated with the occurrence of HIs. The presence of HIs was scored semi-quantitatively (HI−, HI+) in 102 human donor-pancreata. Islet isolation was performed in 40 cases. Donor and procurement factors were retrospectively analyzed in 94 donors. HIs were found in 54.6% of all donor-pancreata. However, only 4.5% of all islets in the affected pancreata was hyperemic. The affected pancreata contained slightly more endocrine tissue, but produced significantly lower yields. When corrected for other factors known to influence isolation outcome, the presence of HIs and endocrine content were the only factors significantly influencing isolation outcome. Prolonged ICU stay and pre-procurement hypertension were associated with the presence of HIs. This study is a first indication that the presence of HIs in human donor-pancreata are associated with reduced isolation outcomes and suggest an impact of the procurement procedure and pre-procurement hemodynamic status of the donor on the islet quality. It is tempting to speculate that this contributes to the generally experienced difficulties in obtaining sufficient amounts of human islets.

Key words

islet isolation - islet transplantation - pancreas - human - hyperemic

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Correspondence

D. E. HillingMD 

Leiden University Medical

Center

Department of Surgery, P-01-Q

PO Box 9600

2300 RC Leiden

The Netherlands

Phone: +31/71/526 2857

Fax: +31/71/526 6750

Email: d.e.hilling@lumc.nl