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Horm Metab Res 2011; 43(6): 404-409
DOI: 10.1055/s-0031-1277154
Animals

© Georg Thieme Verlag KG Stuttgart · New York

Bone and Cellular Immune System of Multiparous Sows are Insensitive to Ovariectomy and Nutritive Calcium Shortage

W. Sipos1 , E. Kralicek1 , M. Rauner2 , C. J. Duvigneau3 , H. L. Worliczek4 , D. Schamall5 , R. T. Hartl3 , I. Sommerfeld-Stur6 , E. Dall’Ara7 , P. Varga7 , H. Resch8 , I. Schwendenwein9 , P. Zysset7 , P. Pietschmann5
  • 1Clinic for Swine, University of Veterinary Medicine Vienna, Austria
  • 2Department of Medicine III, Technical University of Dresden, Germany
  • 3Institute of Medical Chemistry, University of Veterinary Medicine Vienna, Austria
  • 4Institute of Parasitology, University of Veterinary Medicine Vienna, Austria
  • 5Institute of Pathophysiology, Medical University of Vienna, Austria
  • 6Institute of Animal Breeding and Genetics, University of Veterinary Medicine Vienna, Austria
  • 7Institute of Lightweight Design and Structural Biomechanics, Technical University of Vienna, Austria
  • 8II. Medical Department, Krankenhaus der Barmherzigen Schwestern, Vienna, Austria
  • 9Central Laboratory, University of Veterinary Medicine Vienna, Austria
Further Information

Publication History

received 07.12.2010

accepted 06.04.2011

Publication Date:
09 May 2011 (online)

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Abstract

Research in osteoporosis, which is a complex systemic disease, demands suitable large animal models. In pigs, most research has been done in growing minipigs, which probably are not ideal models for postmenopausal osteoporosis. Therefore, our aim was to analyze the effects of ovariectomy (OVX) and nutritive calcium shortage on multiparous Large White sows. 32 animals were randomly assigned to 4 groups in a cross design with OVX vs. sham and physiological calcium supplementation (0.75% calcium) vs. dietary calcium shortage (0.3% calcium). The observation period was 10 months with blood sampling every 2 months for hematological, immunological, and biochemical bone marker measurements. At the termination of the experiment, animals were sacrificed. Samples of trabecular bone of distal radius, proximal tibia, and sixth lumbar vertebra were subjected to micro-computed tomography imaging and ashed afterwards. Dual X-ray absorptiometry scans of the proximal femora were performed with prepared bones being placed in a water bath for mimicking soft tissue. Analyses of bone marker and cytokine profile kinetics, distribution of leukocyte subpopulations, and morphometrical and densitometrical analyses showed no evidence of any impact of OVX or calcium shortage. In conclusion, the skeleton of adult sows of a conventional breed is seemingly protected from effects of OVX and calcium shortage.

Key words

osteoporosis - osteoimmunology - pig

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Correspondence

Univ.Prof. Dr. W. SiposDipl. ECPHM 

Clinic for Swine

University of Veterinary

Medicine Vienna

Veterinärplatz 1

1210 Vienna

Austria

Phone: +43/1/25077 5206

Fax: +43/1/25077 5297

Email: wolfgang.sipos@vetmeduni.ac.at

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