Int J Sports Med 2011; 32(10): 749-753
DOI: 10.1055/s-0031-1277191
Training & Testing

© Georg Thieme Verlag KG Stuttgart · New York

Maximal Lactate Steady State is Altered in the Heat

C. L. M. de Barros1 , T. T. Mendes1 , 2 , L. Á. C. F. Mortimer1 , H. G. Simões3 , L. S. Prado1 , U. Wisloff4 , E. Silami-Garcia1
  • 1Federal University of Minas Gerais, School of Physical Education, Physiotherapy and Occupational Therapy, Belo Horizonte, Brazil
  • 2UNI-BH, Department of Environmental, Biological and Health Sciences, University Center of Belo Horizonte, Brazil
  • 3Catholic University of Brasilia, Department of Physical Education, Distrito Federal, Brazil
  • 4NTNU, Circulation and Medical Imaging, Trondheim, Norway
Further Information

Publication History

accepted after revision April 10, 2011

Publication Date:
17 May 2011 (online)


The aim of this study was to compare the maximal lactate steady state (MLSS) and ventilatory threshold (VT) under different environments (TEMP: 22°C; and HOT: 40°C; 50% RH). 8 male subjects (age 23.9±2.4 years, body mass 75.9±7.3 kg and VO2max 47.8±4.9 mL·kg−1·min−1) performed a series of tests to determine the peak workload (Wpeak), VT and MLSS on a cycle ergometer. Wpeak was higher in the TEMP as compared to the HOT condition (225±9 W vs. 195±8 W, respectively; p<0.05). The workload at MLSS was higher at 22°C (180±11 W) than 40°C (148±11 W; p<0.05), as well as VT at 22°C (156±9 W) was higher than 40°C (128±6 W). Likewise, the blood lactate concentration at MLSS was higher at 22°C (5.60±0.26 mM) than 40°C (4.22±0.48 mM; p<0.05). The mean of heart rate (HR) was not statistically different between TEMP (168±3 bpm) and HOT (173±3 bpm) at MLSS, despite being different at trials between the 25th and the 30th min of exercise. The HR at VT was significantly higher in HOT (153±4 bpm) as compared to the TEMP (145±2 bpm). Our results suggest that environmental conditions may influence the determination of MLSS and VT. Moreover, VT was appropriate for estimation of the workload at MLSS in the HOT.


  • 1 American College of Sports Medicine (ACSM) .Guidelines for Exercise Testing and Prescription.. Philadelphia: Lippincott Williams & Wilkins; 2009: 400
  • 2 Azevedo LF, Perlingeiro PS, Brum PC, Braga AM, Negrao CE, de Matos LD. Exercise intensity optimization for men with high cardiorespiratory fitness.  J Sports Sci. 2011;  29 555-561
  • 3 Baron B, Noakes TD, Dekerle J, Moullan F, Robin S, Matran R, Pelayo P. Why does exercise terminate at the maximal lactate steady state intensity?.  Br J Sports Med. 2008;  42 528-533
  • 4 Baroni BM, Leal Junior EC. Aerobic capacity of male professional futsal players.  J Sports Med Phys Fitness. 2010;  50 395-399
  • 5 Beneke R. Methodological aspects of maximal lactate steady state – implications for performance testing.  Eur J Appl Physiol. 2003;  89 95-99
  • 6 Beneke R, Leithauser RM, Hutler M. Dependence of the maximal lactate steady state on the motor pattern of exercise.  Br J Sports Med. 2001;  35 192-196
  • 7 Beneke R, Von Duvillard SP. Determination of maximal lactate steady state response in selected sports events.  Med Sci Sports Exerc. 1996;  28 241-246
  • 8 Billat VL, Sirvent P, Py G, Koralsztein JP, Mercier J. The concept of maximal lactate steady state: a bridge between biochemistry, physiology and sport science.  Sports Med. 2003;  33 407-426
  • 9 Binder RK, Wonisch M, Corra U, Cohen-Solal A, Vanhees L, Saner H, Schmid J. Methodological approach to the first and second lactate threshold in incremental cardiopulmonary exercise testing.  Eur J Cardiovasc Prev Rehabil. 2008;  15 726-734
  • 10 Convertino VA, Armstrong LE, Coyle EF, Mack GW, Sawka MN, Senay Jr LC, Sherman WM. American College of Sports Medicine Position Stand: Exercise and fluid replacement.  Med Sci Sports Exerc. 1996;  28 i-vii
  • 11 Dean TM, Perreault L, Mazzeo RS, Horton TJ. No effect of menstrual cycle phase on lactate threshold.  J Appl Physiol. 2003;  95 2537-2543
  • 12 Dekerle J, Baron B, Dupont L, Vanvelcenaher J, Pelayo P. Maximal lactate steady state, respiratory compensation threshold and critical power.  Eur J Appl Physiol. 2003;  89 281-288
  • 13 Denadai BS, Figuera TR, Favaro OR, Gonçalves M. Effect of the aerobic capacity on the validity of the anaerobic threshold for determination of the maximal lactate steady state in cycling.  Braz J Med Biol Res. 2004;  37 1551-1556
  • 14 Ettema G, Loras HW. Efficiency in cycling: a review.  Eur J Appl Physiol. 2009;  106 1-14
  • 15 Faude O, Kindermann W, Meyer T. Lactate threshold concepts: how valid are they?.  Sports Med. 2009;  39 469-490
  • 16 Febbraio MA. Alterations in energy metabolism during exercise and heat stress.  Sports Med. 2001;  31 47-59
  • 17 Febbraio MA, Snow RJ, Stathis CG, Hargreaves M, Carey MF. Effect of heat stress on muscle energy metabolism during exercise.  J Appl Physiol. 1994;  77 2827-2831
  • 18 Fink WJ, Costill DL, Van Handel PJ. Leg muscle metabolism during exercise in the heat and cold.  Eur J Appl Physiol. 1975;  34 183-190
  • 19 Friedmann B, Bauer T, Menold E, Bartsch P. Exercise with the intensity of the individual anaerobic threshold in acute hypoxia.  Med Sci Sports Exerc. 2004;  36 1737-1742
  • 20 Gladden LB. Lactate metabolism: a new paradigm for the third millenium.  J Physiol. 2004;  558 5-30
  • 21 Harriss DJ, Atkinson G. International Journal of Sports Medicine – Ethical Standards in Sport and Exercise Science Research.  Int J Sports Med. 2009;  30 701-702
  • 22 Heck H, Mader A, Hess G, Mucke S, Muller R, Hollmann W. Justification of the 4-mmol/l lactate threshold.  Int J Sports Med. 1985;  6 117-130
  • 23 Holloszy JO. Biochemical adaptations to exercise: aerobic metabolism.  Exerc Sport Sci Rev. 1973;  1 45-71
  • 24 Kuipers H, Verstappen FT, Keizer HA, Geurten P, Van Kranenburg G. Variability of aerobic performance in the laboratory and its physiologic correlates.  Int J Sports Med. 1985;  6 197-201
  • 25 Lacerda AC, Gripp F, Rodrigues LO, Silami-Garcia E, Coimbra CC, Prado LS. Acute heat exposure increases high-intensity performance during sprint cycle exercise.  Eur J Appl Physiol. 2007;  99 87-93
  • 26 Laplaud D, Guinot M, Frave-Juvin A, Flore P. Maximal lactate steady state determination with a single incremental test exercise.  Eur J Appl Physiol. 2006;  96 446-452
  • 27 Marino FE, Mbambo Z, Kortekaas E, Wilson G, Lambert MI, Noakes TD, Dennis SC. Influence of ambient temperature on plasma ammonia and lactate accumulation during prolonged submaximal and self-paced running.  Eur J Appl Physiol. 2001;  86 71-78
  • 28 Mattern CO, Gutilla MJ, Bright DL, Kirby TE, Hinchcliff KW, Devor ST. Maximal lactate steady state declines during the aging process.  J Appl Physiol. 2003;  95 2576-2582
  • 29 Mazzeo RS, Marshall P. Influence of plasma catecholamines on the lactate threshold during graded exercise.  J Appl Physiol. 1989;  67 1319-1322
  • 30 Mocellin R, Heusgen M, Gilden HP. Anaerobic threshold and maximal steady-state blood lactate in prepubertal boys.  Eur J Appl Physiol. 1991;  62 56-60
  • 31 Moquin A, Mazzeo RS. Effect of mild dehydration on the lactate threshold in women.  Med Sci Sports Exerc. 2000;  32 396-402
  • 32 Nielsen B, Savard G, Richter EA, Hargreaves M, Saltin B. Muscle blood flow and muscle metabolism during exercise and heat stress.  J Appl Physiol. 1990;  69 1040-1046
  • 33 Philp A, Macdonald AL, Carter H, Watt PW, Pringle JS. Maximal lactate steady state as a training stimulus.  Int J Sports Med. 2008;  29 475-479
  • 34 Rowell LB, Brengelmann GL, Blackmon JR, Twiss RD, Kusumi F. Splanchnic blood flow and metabolism in heat-stressed man.  J Appl Physiol. 1968;  24 475-484
  • 35 Smolander J, Kolari P, Korhonen O, Ilmarinen R. Aerobic and anaerobic responses to incremental exercise in a thermoneutral and a hot dry environment.  Acta Physiol Scand. 1986;  128 15-21
  • 36 Suriano R, Bishop D. Physiological attributes of triathletes.  J Sci Med Sport. 2010;  13 340-347
  • 37 Swensen TC, Harnish CR, Beitman L, Keller BA. Noninvasive estimation of the maximal lactate steady state in trained cyclists.  Med Sci Sports Exerc. 1999;  31 742-746
  • 38 Tyka A, Palka T, Tyka A, Cisoñ T, Szygula Z. The influence of ambient temperature on power at anaerobic threshold determined based on blood lactate concentration and myoelectric signals.  Int J Occup Med Environ Health. 2009;  22 1-6
  • 39 Van Schuylenbergh R, Vanden Eynde B, Hespel P. Correlations between lactate and ventilatory thresholds and the maximal lactate steady state in elite cyclists.  Int J Sports Med. 2004;  25 403-408
  • 40 Young AJ, Sawka MN, Levine L, Cadarette BS, Pandolf KB. Skeletal muscle metabolism during exercise is influenced by heat acclimation.  J Appl Physiol. 1985;  59 1929-1935
  • 41 Wasserman K, McIlroy MB. Detecting the threshold of anaerobic metabolism in cardiac patients during exercise.  Am J Cardiol. 1964;  14 844-852


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