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Int J Sports Med 2014; 35(07): 544-550
DOI: 10.1055/s-0033-1357162
Physiology & Biochemistry
© Georg Thieme Verlag KG Stuttgart · New York

Exercise Decreases Susceptibility to Homocysteine Seizures: the Role of Oxidative Stress

D. Hrncic
1   Faculty of Medicine, Institute of Medical Physiology “Richard Burian”, University of Belgrade, Serbia
,
A. Rasic-Markovic
1   Faculty of Medicine, Institute of Medical Physiology “Richard Burian”, University of Belgrade, Serbia
,
J. Lekovic
1   Faculty of Medicine, Institute of Medical Physiology “Richard Burian”, University of Belgrade, Serbia
,
D. Krstic
2   Faculty of Medicine, Department of Medical Chemistry, University of Belgrade, Serbia
,
M. Colovic
3   Institute of Nuclear Sciences Vinca, University of Belgrade, Serbia
,
D. Macut
4   Faculty of Medicine, Institute of Endocrinology, Diabetes and Metabolic Diseases, University of Belgrade, Serbia
,
V. Susic
5   Department of Medical Sciences, Serbian Academy of Sciences and Arts, Belgrade, Serbia
,
D. Djuric
1   Faculty of Medicine, Institute of Medical Physiology “Richard Burian”, University of Belgrade, Serbia
,
O. Stanojlovic
1   Faculty of Medicine, Institute of Medical Physiology “Richard Burian”, University of Belgrade, Serbia
› Author Affiliations
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Publication History



accepted after revision 02 September 2013

Publication Date:
13 November 2013 (online)

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Abstract

The aim of the study was to examine the effects of chronic exercise training on seizures induced by homocysteine thiolactone (HCT) in adult rats. Rats were assigned to: sedentary control; exercise control; sedentary+HCT; exercise+HCT group. Animals in the exercise groups ran 30 min daily on a treadmill for 30 consecutive days (belt speed 20 m/min), while sedentary rats spent the same time on the treadmill (speed 0 m/min). On the 31st day, the HCT groups received HCT (8.0 mmol/kg), while the control groups received vehicle. Afterwards, convulsive behavior and EEG activity were registered. Lipid peroxidation, superoxide dismutase (SOD) and catalase (CAT) activity were ascertained in the rat hippocampus. No signs of seizures were registered in sedentary and exercise control rats. Seizure latency was increased, while number of seizure episodes and spike-and-wave discharges (SWD) in EEG were decreased in the exercise+HCT compared to the sedentary+HCT group. Seizure incidence, the severity thereof and duration of SWDs were not significantly different between these groups. Exercise partly prevented increase of lipid peroxidation and decrease of the SOD and CAT activity after HCT administration. These results indicate beneficial effects of exercise in model of HCT-induced seizures in rats, what could be, at least in part, a consequence of improved antioxidant enzymes activity.

Key words

physical activity - seizure behavior - homocysteine thiolactone - EEG - rats
 

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