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J Neurol Surg A Cent Eur Neurosurg 2015; 76(01): 20-24
DOI: 10.1055/s-0034-1371513
Original Article
Georg Thieme Verlag KG Stuttgart · New York

Effects of Intrathecal Caffeic Acid Phenethyl Ester and Methylprednisolone on Oxidant/Antioxidant Status in Traumatic Spinal Cord Injuries

Cuneyt Gocmez
1   Department of Neurosurgery, University of Dicle, Diyarbakir, Turkey
,
Feyzi Celik
2   Department of Anesthesiology, University of Dicle, Diyarbakir, Turkey
,
Kagan Kamasak
1   Department of Neurosurgery, University of Dicle, Diyarbakir, Turkey
,
Metin Kaplan
3   Department of Neurosurgery, University of Fırat, Elazığ, Turkey
,
Ertugrul Uzar
4   Department of Neurology, University of Dicle, Diyarbakir, Turkey
,
Adalet Arıkanoglu
4   Department of Neurology, University of Dicle, Diyarbakir, Turkey
,
Osman Evliyaoglu
5   Department of Biochemistry, University of Dicle, Diyarbakir, Turkey
› Author Affiliations
Further Information

Publication History

01 March 2013

13 January 2014

Publication Date:
28 May 2014 (online)

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Abstract

Purpose To examine the effect of intrathecally given caffeic acid phenethyl ester (CAPE) on peroxidation and total oxidant and antioxidant systems, and the effect of intrathecally given methylprednisolone (MP) in spinal cord injury (SCI) models.

Materials and Methods Four groups of 10 rats were formed: (1) Laminectomy, intrathecal saline injection, no SCI (sham: S); (2) Laminectomy, intrathecal saline injection, SCI (control: SCI); (3) Laminectomy, intrathecally given single dose of 3 mg/kg MP, SCISCI (SCI + MP). 4) Laminectomy, intrathecally given single dose of 1 µg/kg CAPE, SCI (SCI + CAPE). Malondialdehyde (MDA), total oxidant activity (TOA), total antioxidant capacity (TAC), superoxide dismutase (SOD), and glutathione peroxidase (GPx) values in the spinal cord tissue were evaluated.

Results When group S and group SCI were compared, MDA, TOA, and SOD parameters increased post-SCI (p < 0.01). When compared with group SCI, it was observed that CAPE and MP decreased the MDA, TOA, and SOD levels (p < 0.01). This decrease was more pronounced in the SCI + CAPE group. When group S and group SCI were compared, a statistically substantial decrease was observed in the post-SCI TAC levels. When compared with group SCI, it was shown that CAPE and MP treatment substantially increased TAC levels (p < 0.001).

Conclusion Intrathecal injection of both CAPE and MP inhibits lipid peroxidation and increase of oxidants in SCIs.

Keywords

spinal cord injury - caffeic acid phenethyl ester - lipid peroxidation - intrathecal
 

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