Abstract
In this study[*], it was aimed to observe, genotoxic effects of antituberculosis drugs and combinations
on rats. Animals were treated with 31.5 mg/kg isoniazid (INH), 54 mg/kg rifampicin
(RIF), 189 mg/kg pyrazinamide (PYR), 100 mg/kg ethambutol(ETA), INH+RIF+PYR (MIX1)
and INH+RIF+PYR+ETA (MIX2) mixtures applied via gavage for 90 days. At the end of
the study, blood, liver and kidney samples were taken and evaluated by Comet and Micronucleus
techniques. Compared to control group, head intensity decreased, tail intensity and
tail migration increased on experiment groups in blood samples. Head intensity of
PYR and mixture groups decreased, tail intensity of PYR and mixture groups increased
and tail migration of PYR, ETA and mixture groups increased in liver samples. Head
intensity decreased and tail intensity increased of INH, RIF, ETA and MIX1 group;
tail migration increased of MIX1 group in kidney samples. Compared to control group,
micronucleus rate of ETA, RIF and MIX 2 groups increased in experiment groups. In
conclusion antituberculosis drugs and their mixtures applied for 90 days causes to
double strand break of DNA damage at different degrees in blood, kidney and liver
cells in rats.
Key words
antituberculosis drug - genotoxicity - rat - comet - micronucleus
