Download PDF
Int J Sports Med 2015; 36(02): 93-100
DOI: 10.1055/s-0034-1385877
Physiology & Biochemistry
© Georg Thieme Verlag KG Stuttgart · New York

The Effect of Aerobic Exercise and Macrothele raven Venom on Tumor-bearing Mice

Z. J. Sheng
1   College of Physical Education, Hunan Normal University, Changsha, China
,
C. J. Qin
1   College of Physical Education, Hunan Normal University, Changsha, China
,
C. W. Wei
1   College of Physical Education, Hunan Normal University, Changsha, China
,
L. C. Miao
2   Central Hospital of Shaoyang, Shaoyang, China
,
Z. G. Hua
2   Central Hospital of Shaoyang, Shaoyang, China
,
C. Rui
2   Central Hospital of Shaoyang, Shaoyang, China
,
L. Lin
1   College of Physical Education, Hunan Normal University, Changsha, China
,
W. M. Cai
2   Central Hospital of Shaoyang, Shaoyang, China
› Author Affiliations
Further Information

Publication History



accepted after revision 04 June 2014

Publication Date:
25 September 2014 (online)

Also available at
    Permissions and Reprints

Abstract

Liver cancer is one of the most common cancers in the world. Macrothele raven venom, a complicated mixture of neurotoxic peptides, proteins and low molecular weight material, has antitumor properties, but its mechanism of action is unknown. Moderate exercise has been shown to shrink tumors and cause a remarkable reduction in the tumor growth rate. In this study, we examined the antitumor effect of Macrothele raven venom in combination with exercise on tumor-bearing mice. Our results demonstrate that aerobic exercise in combination with venom administered at different doses was much more effective in a mouse H22 hepatoma model compared to separate administration of the 2 treatments. The underlying mechanism of this effect may be related to the expression of various tumor suppressor factors.

Key words

aerobic exercise - Macrothele raven venom - tumor-bearing mice
 

  • References

  • 1 Bai J, Cederbaum AI. Cycloheximide protects HepG2 cells from serum withdrawal induced apoptosis by decreasing p53 and phosphorylated p53 levels. J Pharmacol Exp Ther 2006; 319: 1435-1443
    CrossrefPubMedGoogle Scholar
  • 2 Blum HE. Molecular targets for prevention of hepatocellular carcinoma. Dig Dis 2002; 20: 81-90
    CrossrefPubMedGoogle Scholar
  • 3 Evangelista FS, Brum PC, Krieger JE. Duration-controlled swimming exercise training induces cardiac hypertrophy in mice. Braz J Med Biol Res 2003; 36: 1751-1759
    CrossrefPubMedGoogle Scholar
  • 4 Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM. Estimates of worldwide burden of cancer in 2008: Globocan 2008. Int J Cancer 2010; 127: 2893-2917
    CrossrefPubMedGoogle Scholar
  • 5 Harriss DJ, Atkinson G. Ethical standards in sports and exercise science research. Int J Sports Med 2013; 34: 1025-1028
    Article in Thieme ConnectPubMedGoogle Scholar
  • 6 Hidalgo M, Rowinsky EK. The rapamycin-sensitive signal transduction pathway as a target for cancer therapy. Oncogene 2000; 19: 6680-6686
    CrossrefPubMedGoogle Scholar
  • 7 Huang J, Manning BD. The TSC1-TSC2 complex: a molecular switchboard controlling cell growth. Biochem J 2008; 412: 179-190
    CrossrefPubMedGoogle Scholar
  • 8 Gao L, Shen JB, Sun J, Shan BE. Effect of the venom of the spider Macrothele raven on the expression of p21 gene in HepG2 cells. Sheng Li Xue Bao 2007; 59: 58-62
    PubMedGoogle Scholar
  • 9 Gao L, Shan BE, Chen J, Liu JH, Song DX, Zhu BC. Effects of spider Macrothele raven venom on cell proliferation and cytotoxicity in HeLa cells. Acta Pharmacol Sin 2005; 26: 369-376
    CrossrefPubMedGoogle Scholar
  • 10 Lin L, Cai WM, Qin CJ, Miao LC, Yun LT, Hua Y, Weilin L. Intervention of TLR4 signal pathway cytokines in severe liver injury with obstructive jaundice in rats. Int J Sports Med 2012; 33: 572-579
    Article in Thieme ConnectPubMedGoogle Scholar
  • 11 Lötzerich H, Fehr HG, Appell HJ. Potentiation of cytostatic but not cytolytic activity of murine macrophages after running stress. Int J Sports Med 1990; 11: 61-65
    Article in Thieme ConnectPubMedGoogle Scholar
  • 12 Maehama T, Dixon JE. The tumor suppressor, PTEN/MMAC1, dephosphorylates the lipid second messenger, phosphatidylinositol 3, 4, 5-trisphosphate. J Biol Chem 1998; 273: 13375-13378
    CrossrefPubMedGoogle Scholar
  • 13 Meng J, Yang M, Jia F, Kong H, Zhang W, Wang C, Xing J, Xie S, Xu H. Subcutaneous injection of water-soluble multi-walled carbon nanotubes in tumor-bearing mice boosts the host immune activity. Nanotechnology 2010; 21: 145104
    CrossrefPubMedGoogle Scholar
  • 14 Ruggeri B, Singh J, Gingrich D, Angeles T, Albom M, Yang S, Chang H, Robinson C, Hunter K, Dobrzanski P, Jones-Bolin S, Pritchard S, Aimone L, Klein-Szanto A, Herbert JM, Bono F, Schaeffer P, Casellas P, Bourie B, Pili R, Isaacs J, Ator M, Hudkins R, Vaught J, Mallamo J, Dionne C. CEP27055: A Novel, orally active pan inhibitor of vascular endothelial growth factor receptor tyrosine kinases with potent antiangiogenic Activity and antitumor efficacy in preclinical models. Cancer Res 2003; 63: 5978-5991
    PubMedGoogle Scholar
  • 15 Steck PA, Pershouse MA, Jasser SA, Yung WK, Lin H, Ligon AH, Langford LA, Baumgard ML, Hattier T, Davis T, Frye C, Hu R, Swedlund B, Teng DH, Tavtigian SV. Identification of a candidate tumor suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat Genet 1997; 15: 356-362
    CrossrefPubMedGoogle Scholar
  • 16 Suetsugu A, Katz M, Fleming J, Truty M, Thomas R, Saji S, Moriwaki H, Bouvet M, Hoffman RM. Non-invasive fluorescent-protein imaging of orthotopic pancreatic-cancer-patient tumor graft progression in nude mice. Anticancer Res 2012; 32: 3063-3067
    PubMedGoogle Scholar
  • 17 Zeng XZ, Xiao QB, Liang SP. Purification and characterization of raven toxin-I and raven toxin-III, two neurotoxic peptides from the venom of the spider Macrothele raven. Toxicon 2003; 41: 651-656
    CrossrefPubMedGoogle Scholar
  • 18 Zhai Y, Lu ZJ. Effect of thalidomide on tumor growth in mouse hepatoma H22 model. Ai Zheng 2003; 22: 1301-1306
    PubMedGoogle Scholar