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Int J Sports Med 2015; 36(03): 262-270
DOI: 10.1055/s-0034-1389904
Immunology
© Georg Thieme Verlag KG Stuttgart · New York

Systemic LPS and Inflammatory Response during Consecutive Days of Exercise in Heat

M. D. Barberio
1   Children’s National Medical Center, Center for Genetic Medicine Research, Washington, D.C., United States
4   School of Kinesiology, Auburn University, Auburn, AL, United States
,
D. J. Elmer
2   Department of Kinesiology, Berry College, Mount Berry, GA, United States
4   School of Kinesiology, Auburn University, Auburn, AL, United States
,
R. H. Laird
1   Children’s National Medical Center, Center for Genetic Medicine Research, Washington, D.C., United States
3   Department of Exercise Science & Physical Education, McDaniel College, Westminster, MD, United Sates
,
K. A. Lee
4   School of Kinesiology, Auburn University, Auburn, AL, United States
,
B. Gladden
4   School of Kinesiology, Auburn University, Auburn, AL, United States
,
D. D. Pascoe
4   School of Kinesiology, Auburn University, Auburn, AL, United States
› Author Affiliations
Further Information

Publication History



accepted after revision 25 July 2014

Publication Date:
19 December 2014 (online)

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Abstract

This investigation studied circulating LPS activity, potential intestinal damage, and the systemic inflammatory response (SIR) during the exercise heat acclimation process. 8 healthy males (Age=24±3 years) ran in a hot environment on 5 consecutive days until core temperature (Tc) was elevated 2°C above rest. Plasma was obtained pre-, post-, 1 h post-, and 3 h post-exercise on the 1st, 3rd, and 5th day of exercise and analyzed for TNF-α, IL-6, IL-10, IL-1ra, LPS, and intestinal fatty acid-binding protein (I-FABP). Plasma LPS (1.1 EU·ml−1±0.1 vs. 0.7 EU·ml−1±0.03; P<0.01) and I-FABP (930.7 pg·ml−1±149.0 vs. 640.2 pg·ml−1±125.0; P<0.001) were significantly increased post-exercise each. The SIR remained largely unchanged during the study except for TNF-α. Plasma TNF-α was significantly lower on Day 5 at 1 h (3.2 pg·ml−1±0.6 vs. 4.5 pg·ml−1±0.8; P=0.01) and 3 h (3.6 pg·ml−1±0.8 vs. 4.8 pg·ml−1±0.9; P=0.05) post-exercise as compared to Day 1. Findings indicate that adaptations to exercise in the heat resulting in reductions of intestinal damage and plasma LPS activity require longer time periods in moderately trained males.

Key words

endotoxin - hyperthermia - cytokines
 

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