Suppression of Inducible Nitric Oxide Synthase Pathway by 7-Deacetylgedunin, a Limonoid from Xylocarpus sp.

Chanin Sarigaputi; Nuanpan Sangpech; Tanapat Palaga; Khanitha Pudhom

doi:10.1055/s-0034-1396308

Planta Medica, Table of Contents

Planta Med 2015; 81(04): 312-319
DOI: 10.1055/s-0034-1396308

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Suppression of Inducible Nitric Oxide Synthase Pathway by 7-Deacetylgedunin, a Limonoid from Xylocarpus sp.

Authors

Chanin Sarigaputi

¹Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
Nuanpan Sangpech

²Medical Microbiology Interdisciplinary Program, Graduate School, Chulalongkorn University, Bangkok, Thailand
Tanapat Palaga

³Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
Khanitha Pudhom

⁴Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand

Abstract

In this study, limonoids isolated from Xylocarpus plants were tested for their in vitro anti-inflammatory effects. The results demonstrated that only 7-deacetylgedunin (1), a gedunin-type limonoid, significantly inhibited lipopolysaccharide- and interferon-γ-stimulated production of nitric oxide in murine macrophage RAW 264.7 cells. The suppression of nitric oxide production by 1 was correlated with the downregulation of mRNA and protein expression of inducible nitric oxide synthase. Mechanistic studies revealed that the transcriptional activity of nuclear factor-κB, IκBα degradation, and the activation of mitogen-activated protein kinases, stimulated with lipopolysaccharide and interferon-γ, were suppressed by 1.

Key words

gedunin - limonoid - anti-inflammatory effect - nitric oxide - iNOS

Full Text

References

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